针刺对家兔脑出血急性期超氧化物歧化酶、过氧化脂质的分时影响

目的 探讨针刺治疗脑出血机理。方法 将54只兔随机分为对照组、模型组和针刺组。采用脑内注血法造成动脉粥样硬化兔脑出血模型。选水沟、风府、曲泽、内关、三阴交、血海、太溪穴。观察针刺对急性脑出血脑组织超氧化物歧化酶(SOD)和过氧化脂质(LPO)影响。结果 模型组和针刺组SOD较对照组显著降低，并以模型组降低最为明显。脑出血后24h、72h针刺组SOD均明显高于模型组。脑出血后72h，模型组SOD较24h进一步降低；针刺组较24h明显升高。脑出血后1星期，模型组SOD较72h有所升高，但仍显著低于针刺组；针刺组SOD明显高于24h。模型组、针刺组LPO较对照组显著升高，并以模型组升高最为明显。脑出血后24h和72h，针刺组LPO均明显低于模型组。脑出血后72h，模型组、针刺组LPO较24h升高。脑出血1星期，针刺组和模型组间有极显著性差异；模型组、针刺组LPO较24h均明显降低，且明显低于72h水平。结论 在脑出血急性期，针刺可显著增强脑SOD活性，显著降低LPO含量，能稳定地增强脑出血兔抗自由基酶活性并降低脑出血后自由基脂质过氧化反应。

Effects of Acupuncture on SOD and LPO in Rabbits of Acute Cerebral Hemorrhage

Purpose To research the mechanism of acupuncture on acute cerebral hemorrhage (ACH). Methods On the basis of atherosclerosis cerebral hemorrhage rabbit model built by feeding ketogenic forage and injection blood into brain, we acupunctured Suigou (GV 26),Fengfu (GV 16),Quze (PC 3),Neiguan (PC 6),Sanyingjiao (SP 6),Xuehai (SP 10) and Taixi (KI 3) to observe the effect of acupuncture on several index of ACH case in Control, Model and Acup groups.Results In the 3 segments, compared with Control the SOD activity decreased obviously in Model and Acup (P< 0.01), and especially the Model 24 h and 72 h after cerebral hemorrhage (CH), the SOD activity in Acup was obviously higher than that in Model (P< 0.01). Compared with SOD activity at 24 h after CH, it was further decreased at 72 h in Model (P< 0.01), while it was obviously elevated in Acup (P< 0.05). Compared with SOD activity at 72 h after CH, it was elevated at 1 week after CH in Model, while obviously less than that in Acup (P< 0.01) and the SOD activity in Acup was obviously higher than that at 24 h (P< 0.05). In the 3 segments, compared with Control, the LPO increased obviously in Model and Acup (P< 0.01), and especially the Model. 24 h and 72 h after CH, the LPO in Acup was obviously lower than that in Model (P< 0.01). Compared with LPO at 24 h after CH, it was further increased at 72 h in Model and Acup (P< 0.01). Compared with LPO at 72 h after CH, it was decrcased at 1 week after CH in Model and Acup; the LPO in Acup is obviously less than that in Model (P< 0.01); the LPO in Model and Acup was obviously lower than that at 24 h (P< 0.05 and P< 0.01). Conclusion Compared with Model, acupuncture applied for ACH early could significantly improve the activity of SOD and reduce the content of LPO in brain and could protect the function of brain.