血管内皮生长因子和基质金属蛋白酶-9与子痫前期的相关性研究 |
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引用本文: | 吴桂梅,刘洪玲,王文杰,李冀云,曹艳敏. 血管内皮生长因子和基质金属蛋白酶-9与子痫前期的相关性研究[J]. 中国计划生育和妇产科, 2016, 0(10). DOI: 10.3969/j.issn.1674-4020.2016.10.19 |
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作者姓名: | 吴桂梅 刘洪玲 王文杰 李冀云 曹艳敏 |
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作者单位: | 沧州市中心医院产三科, 河北 沧州,061000 |
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摘 要: | 目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)与子痫前期发病的相关性。方法纳入河北省沧州市中心医院2013年1月至2014年11月确诊为子痫前期的患者100例,其中轻度子痫前期的患者51例,重度子痫前期患者49例,同时纳入同期分娩的健康孕妇50例作为对照组。采用Western blotting、反转录酶-聚合酶链反应分子生物学方法检测3组患者胎盘组织中VEGF、MMP-9的表达水平;采用酶联免疫吸附方法检查患者血清及尿液中VEGF、MMP-9的水平。结果轻、重度子痫前期组患者的胎盘组织中VEGF、MMP-9的表达水平均低于正常对照组,且组间相比差异有统计学意义(P0.05);轻、重度子痫前期组患者血清及尿液中VEGF、MMP-9的水平均低于正常对照组,且组间相比差异有统计学意义(P0.05)。结论 VEGF、MMP-9参与了子痫前期的病理生理改变,且与病情的发生及轻重程度有密切相关性。
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关 键 词: | 血管内皮生长因子(VEGF) 基质金属蛋白酶-9(MMP-9) 子痫前期 相关性 |
The correlation of preeclampsia and vascular endothelial growth factor and matrix metalloproteinase-9 |
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Abstract: | Objective To investigate the correlation of vascular endothelial growth factor ( VEGF ) , matrix metalloproteinase-9 (MMP-9) and incidence of preeclampsia .Methods 100 patients diagnosed with preeclampsia in Cangzhou Central hospital from January 2013 to November 2014 were selected,including 51 cases of mild pre-eclampsia and 49 cases severe preeclampsia, while 50 healthy pregnant women during the same period were set as the control group .Using Western blotting , RT-PCR molecular biology methods to detect VEGF and MMP -9 expression levels in patients ’ placent of three groups;using ELISA method to check VEGF and MMP-9 serum levels of serum and urine .Results The expression levels of VEGF and MMP -9 in placental tissue in patients with preeclampsia were lower than those in the control group , with a statistically significant difference (P<0.05);the levels of VEGF and MMP-9 in serum and urine in patients with preeclampsia were lower than those in the control group , with a significant difference ( P<0.05).Conclusion VEGF and MMP -9 involve in the pathophysiology of preeclampsia , and have a close correlation with the occurrence and severity of the disease . |
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Keywords: | vascular endothelial growth factor (VEGF) matrix metalloproteinase-9 (MMP-9) preeclampsia correlation |
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