Fermentative production of extracellular amylase from novel amylase producer,Tuber maculatum mycelium,and its characterization |
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Authors: | Dattatray Bedade Jan Deska Sandip Bankar Samir Bejar Rekha Singhal Salem Shamekh |
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Affiliation: | 1. Department of Food Engineering and Technology, Institute of Chemical Technology, Mumbai, India;2. ft13dk.bedade@pg.ictmumbai.edu.in;4. Department of Chemistry, School of Chemical Technology, Aalto University, Aalto, Finland;5. Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, Aalto, Finland;6. Laboratory of Microbial Biotechnology and Engineering Enzymes, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia;7. Juva Truffle Center, Juva, Finland |
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Abstract: | AbstractTruffles are symbiotic hypogeous edible fungi (form of mushroom) that form filamentous mycelia in their initial phase of the growth cycle as well as a symbiotic association with host plant roots. In the present study, Tuber maculatum mycelia were isolated and tested for extracellular amylase production at different pH on solid agar medium. Furthermore, the mycelium was subjected to submerged fermentation for amylase production under different culture conditions such as variable carbon sources and their concentrations, initial medium pH, and incubation time. The optimized conditions after the experiments included soluble starch (0.5% w/v), initial medium pH of 7.0, and incubation time of 7 days, at room temperature (22?±?2?°C) under static conditions which resulted in 1.41?U/mL of amylase. The amylase thus obtained was further characterized for its biocatalytic properties and found to have an optimum activity at pH 5.0 and a temperature of 50?°C. The enzyme showed good thermostability at 50?°C by retaining 98% of the maximal activity after 100?min of incubation. The amylase activity was marginally enhanced in presence of Cu2+ and Na+ and slightly reduced by K+, Ca2+, Fe2+, Mg2+, Co2+, Zn2+, and Mn2+ ions at 1?mM concentration. |
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Keywords: | Amylase biochemical characterization starch truffle Tuber maculatum |
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