基于网络药理学研究妇科千金片/胶囊治疗盆腔炎性疾病后遗症的作用机制

目的 通过网络药理学结合文献验证的方法,探讨妇科千金片/胶囊治疗盆腔炎性疾病后遗症(sequelae of pelvic in-flammatory disease,SPID)的作用机制.方法 基于TCMSP、TCMID等数据库及文献收集妇科千金片/胶囊的化学成分,筛选后获得活性成分及对应靶点;采用GeneGards数...

The mechanism of Fuke Qianjin Tablet/Capsule in treating sequelae of pelvic inflammatory disease based on network pharmacology

Objective To investigate the effect of Fuke Qianjin Tablet/Capsule on sequelae of pelvic inflammatory disease(SPID) by network pharmacology combined with literature validation. Methods The chemical constituents of Fuke Qianjin Tablet/Capsule were collected based on TCMSP, TCMID and other databases and literatures, and the active constituents and corresponding targets were obtained after screening. GeneGards database was used to obtain SPID related targets. STRING database was used to analyze the protein interactions of the intersection targets. DAVID database was used for gene function and pathway enrichment analysis. Cytoscape software was used to construct an “active component-target-pathway” network, which was evaluated in conjunction with the literature. Results 88 active components were screened from Fuke Qianjin Tablet/Capsule, with 597 corresponding targets and 169 intersection targets with SPID. Wogonin, berberine, luteolin and other 77 chemical components may be the main active components of Fuke Qianjin Tablet/Capsule;the 80 targets such as estrogen receptor 1, prostaglandin endoperoxide synthase 2 and nitric oxide synthase 2 may be the key targets for the treatment of SPID by Fuke Qianjin Tablet/Capsule. Nine signaling pathways, including phosphateidylinositol 3 kinase/serine-threoninekinase, hypoxia inducible factor-1 and tumor necrosis factor may be the main pathways for the therapeutic effect of Fuke Qianjin Tablet/Capsule. Conclusion Fuke Qianjin Tablet/Capsule can treat SPID through multi-component and multi-target synergistic therapy, and its mechanism may be related to regulating PI3 K-Akt pathway, improving blood circulation, regulating inflammation, immune response and apoptosis.