| 日本医蛭和天目山蛭4种同工酶的比较研究 |
| |
| 引用本文： | 罗媛媛,姜乃澄.日本医蛭和天目山蛭4种同工酶的比较研究[J].浙江大学学报(理学版),2002,29(1):99-102. |
| |
| 作者姓名： | 罗媛媛 姜乃澄 |
| |
| 作者单位： | 浙江大学,生命科学学院,浙江,杭州,310028 |
| |
| 基金项目： | 浙江省自然科学基金资助项目 (No. 392 15 4) |
| |
| 摘 要： | 采用垂直平板聚丙烯酰胺凝胶电泳对日本医蛭和天目山蛭的酯酶（ES）、苹果酸脱氢酶（MDH）、过氧化物酶（POX）、乳酸脱氢酶（LDH）4种同工酶共计11个位点的15个乘闰基因进行了分析，4种同工酶基因在2种蛭中表达程度以及LDH同工酶酶谱相似，但是日本医蛭比天目山蛭ES同工酶多2个位点，3个基因；MDH少1个基因；POX同工酶多2个位点，2个基因；LDH同工酶等位基因表达的亚单位类型和数目不同。同时分析了冬眠与非冬眠期日本医蛭LDH同工酶活性差异的原因，为在分子水平研究蛭类分类、不同环境温度下酶的活性变化等提供和积累新的生物学资料。
|
| 关 键 词： | 日本医蛭 天目山蛭 同工酶 基因表达 冬眠 酯酶 过氧化物酶 乳化酸脱氢酶 MDH |
| 文章编号： | 1008-9497(2002)01-0099-04 |
Comparative studies on four isozymes in Hirudo nipponia and Haemadipa tianmushana . |
| |
| LUO Yuan\|yuan,JIANG Nai\|cheng.Comparative studies on four isozymes in Hirudo nipponia and Haemadipa tianmushana .[J].Journal of Zhejiang University(Sciences Edition),2002,29(1):99-102. |
| |
| Authors: | LUO Yuan\|yuan JIANG Nai\|cheng |
| |
| Abstract: | Using vertical polyacrylamide gel electrophoresis. Four isozymes in \%Hirudo nipponia\% and \%Haemadipa tianmushana\% were compared. Esterase(ES), malic dehydrogenase(MDH), peroxydase(POX) and lactate dehydrogenase(LDH) were found to be determined by 15 alleles of 11 loci in them. The expressivity of the 4 isozymes and the patterns of LDH of the two leeches were similar. But the patterns of ES, MDH and POX were different between them. The systematic position of Hirudinea was discussed on a molecular level. The reasons of the difference of LDH activity in \%Hir. nipponia\% during hibernation and non\|hibernation were also analyzed. |
| |
| Keywords: | Hirudo nipponia Haemadipa tianmushana\% isozyme expression of gene hibernation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《浙江大学学报(理学版)》浏览原始摘要信息 |
|
点击此处可从《浙江大学学报(理学版)》下载全文 |
