| Vascular Endothelial Growth Factor165-regulated Nasopharyngeal Carcinoma Cell Lines Invasion and Migration Involve Expression and Activation of Matrix Metalloproteinase-2 |
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| 引用本文： | 王彦君,孔维佳,乐建新,孙大为,李伟,姚琪,孙宇,董继华. Vascular Endothelial Growth Factor165-regulated Nasopharyngeal Carcinoma Cell Lines Invasion and Migration Involve Expression and Activation of Matrix Metalloproteinase-2[J]. 华中科技大学学报(医学英德文版), 2006, 26(5): 621-624. DOI: 10.1007/s11596-006-0538-z |
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| 作者姓名： | 王彦君 孔维佳 乐建新 孙大为 李伟 姚琪 孙宇 董继华 |
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| 作者单位： | [1]Department of Otorhinolaryngology,Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China [2]Laboratory of Virology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| 基金项目： | This project was supported by grants from National Excel lent Young Scientists Foundation of China （No.39925035） and the Major Clinical Project of Ministry of Health （No.22012332）. |
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| 摘 要： | The effect of vascular endothelial growth factor （VEGF） overexpression on matrix metalloproteinase-2 （MMP-2） in nasopharyngeal carcinoma （NPC） cells in vitro and the possible mechanism involved were investigated, and the correlation between the expression of VEGF and MMP-2 in NPC evaluated. The NPC cells were transfected with PAd-trackVEGF165 plasmid. The expression levels of VEGF and MMP-2 mRNA and protein in NPC cells were detected by semi-quantitative RT-PCR and Western blot respectively. It was found that the expression of VEGF and MMP-2 mRNA and protein was significantly increased in NPC cells after transfection of VEGF 165. It was concluded that the expression of VEGF was correlated to the in vitro invasion of NPC cells, and the induction of MMP-2 by VEGF was a key process of NPC cell invasion.
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| 关 键 词： | 病理 治疗 内皮生长因子 鼻咽癌 |
| 收稿时间： | 2006-07-06 |
Vascular endothelial growth factor165-regulated nasopharyngeal carcinoma cell lines invasion and migration involve expression and activation of matrix metalloproteinase-2 |
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| Yanjun Wang M.D., Ph.D.,Weijia Kong,Jianxin Yue,Dawei Sun,Wei Li,Qi Yao,Yu Sun,Jihua Dong. Vascular endothelial growth factor165-regulated nasopharyngeal carcinoma cell lines invasion and migration involve expression and activation of matrix metalloproteinase-2[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2006, 26(5): 621-624. DOI: 10.1007/s11596-006-0538-z |
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| Authors: | Yanjun Wang M.D. Ph.D. Weijia Kong Jianxin Yue Dawei Sun Wei Li Qi Yao Yu Sun Jihua Dong |
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| Affiliation: | 1. Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2. Laboratory of Virology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Summary The effect of vascular endothelial growth factor (VEGF) overexpression on matrix metalloproteinase-2 (MMP-2) in nasopharyngeal carcinoma (NPC) cells in vitro and the possible mechanism involved were investigated, and the correlation between the expression of VEGF and MMP-2 in NPC evaluated. The NPC cells were transfected with PAd-trackVEGF165 plasmid. The expression levels of VEGF and MMP-2 mRNA and protein in NPC cells were detected by semi-quantitative RT-PCR and Western blot respectively. It was found that the expression of VEGF and MMP-2 mRNA and protein was significantly increased in NPC cells after transfection of VEGF165. It was concluded that the expression of VEGF was correlated to the in vitro invasion of NPC cells, and the induction of MMP-2 by VEGF was a key process of NPC cell invasion. This project was supported by grants from National Excellent Young Scientists Foundation of China (No. 39925035) and the Major Clinical Project of Ministry of Health (No. 22012332). |
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| Keywords: | nasopharyngeal carcinoma cells vascular endothelial growth factor matrix metalloproteinase-2 gene transfection |
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