酶解-膜分离组合制备ACE抑制肽

测试了膜分离操作压力、温度、pH值、料液体积浓度等操作参数对酶活力、血管紧张素转换酶(ACE)抑制肽分离的影响,并建立分段酶解、分级膜分离组合模式实现从扇贝裙边酶解产物中分离ACE抑制肽.结果表明:膜分离操作操作参数对酶活力、ACE抑制肽分离均有较大影响;二段酶解-二级膜分离组合模式制备及分离ACE抑制肽的得率最高,为5.73%,其IC50为0.088mg/ml;一段酶解-二级膜分离组合模式制备及分离ACE抑制肽的得率为3.19%,其IC50为0.081mg/ml;一段酶解-单级膜分离组合模式制备及分离ACE抑制肽的得率只有2.31%,其IC50为0.078mg/ml.

Preparation of ACE Inhibitor Peptides with Combination System of Hydrolysis-Membrane Separation

Effects of separation pressure, temperature, pH and the ratio of E/s on enzymic activity and separation of ACE (angiotensin converting enzyme) inhibitory peptides were investigated. The combination system of hydrolysis-membrane separation to separate ACE inhibitory peptides from hydrolysates was established. The results showed that the effects of separation parameters of membrane separation on enzymic activity and separation of ACE inhibitory peptides are prominent. The combination system of two-times hydrolysis-two-ranks membrane separation possesses the high yield of ACE inhibi-tory peptides as 5.73%, while IC50 (half inhibit concentration) as 0.088 mg/ml. The yields of ACE inhibitory peptides of the two combination system. Such as were one-time hydrolysis-two-ranks membrane separation and of one-time hydrolysis-one-rank membrane separation, are 3.19% and 2.31% respectively, while IC50 values are 0.081 mg/ml and 0.078 mg/ml, respectively.