| HPLC法测定人体内咪达唑仑血药浓度 |
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| 引用本文： | 赵丽,阎克里,张勤功,邓丽云,耿力,赵真英,赵嘉训. HPLC法测定人体内咪达唑仑血药浓度[J]. 中国现代药物应用, 2009, 3(5) |
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| 作者姓名： | 赵丽 阎克里 张勤功 邓丽云 耿力 赵真英 赵嘉训 |
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| 作者单位： | 1. 山西省肿瘤医院药物检测室,030001
2. 山西省肿瘤医院肿瘤医院麻醉科,030001 |
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| 摘 要： | 目的建立人体血浆中同时测定咪达唑仑HPLC测定法。方法采用HPLC法,色谱柱为Hypersil ODS C18柱(250mm×4.6mm,5μm),流动相为甲醇:水(60:40)(含0.1%二乙胺,0.05%磷酸溶液),流速为1.0ml/min,柱温为室温,检测波长225nm。结果咪达唑仑在0.25~4μg/ml内呈良好的线性关系(r=0.9997),咪达唑仑最低检测浓度为50ng/ml,回收率96.81%~100.98%,萃取回收率87.69%~92.99%。日内、日间RSD(n=3)均小于5.89%。结论本方法简便、准确,可靠,可用于人体血浆中测定咪达唑仑的浓度。
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| 关 键 词： | 咪达唑仑 高效液相色谱法 |
Determination of midazolam in human plasma by reversed-phase high performance liquid chromatography |
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| Affiliation: | ZHAO Li; YAN Ke-li; ZHANG Qin-gong; et al.Department of drug detection; shanxi provincial tumour hospital; |
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| Abstract: | Objective To develop an HPLC method for the determination of midazolam in human plasma.MethodsThe analytical column was packed with Hypersil ODS C18(250 mm×4.6 mm,5 μm),the mobile phase was methanol:water60:40,(containing 0.1%diethyl amine,0.05%phosphoric acid),the flow rate was 1 ml/min,and detection wavelength was set at 225 nm.ResultsThe linear ranges were 0.25~4 μg/ml for midazolam in human plasma,.The correlation coefficients were 0.9997.The midazolam low limit of detection was 50 ng/ml.The method reco... |
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| Keywords: | Midazolam HPLC |
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