实验性骨不连模型的制作

背景目前在探讨骨缺损的物理和药物方法治疗的效果、检验手术方式的有效性、以及评价骨替代材料在骨缺损修复中所起的作用时,尚缺少一种客观的、标准的动物模型.目的为骨不连的实验研究建立一种客观的动物实验模型.设计制作兔骨不连模型的验证性实验.单位深圳市人民医院骨科.材料取普通级纯种新西兰大白兔20只,体质量(2.5±0.5)kg,6～8月龄,雌雄不限.方法实验于1999-05/08在深圳市人民医院动物实验中心完成.选取纯种新西兰大白兔20只,在前臂桡骨中段截除1.5 cm骨段(包括骨膜),骨断端用骨蜡封闭髓腔,10周后经大体标本、病理组织学及X射线检查确定骨不连形成情况.主要观察指标兔前臂桡骨骨缺损模型大体观察及病理组织学和放射学检查结果.结果纳入白兔20只(40侧桡骨)均进入结果分析.①兔骨缺损区大体观察10周后骨缺损区可见纤维瘢痕组织填充,无骨性连接,骨蜡未吸收,骨端硬化,髓腔闭塞,两侧断端有少量骨痂形成,骨缺损范围为0.8～1.2 cm.②兔骨缺损区病理组织学检查光镜下骨断端髓腔封闭,有软骨细胞及骨细胞,细胞呈无序排列,纤维膜覆盖,缺损区为纤维瘢痕组织.③兔骨缺损区放射学检查于10周Z射线拍片见骨缺损区无骨性连接,断端硬化,髓腔封闭,两侧断端有少量骨痂形成,骨痂形成不规则.结论实验所建立的动物模型,具有骨不连的病理改变,符合骨不连的要求,反映了骨不连的一般特征,可以认为是一种可靠而实用的实验性骨不连动物模型.

Preparation of experimental models of osseous nonunion

BACKGROUND:There is lack of an objective and standardized animal model for assessing the therapeutic effect of physical and medication treatment on bone defoct, the effectiveness of operation, as well as the role of bone substitute in the repairing of bone defects.DESIGN:Verified study on the experimental model of bone nonunion in rabbitsSETTING: Department of Orthopaedics in Shenzhen people' s Hospital MATERIALS:Twenty common grade pure New Zealand rabbits of either gender were selected with body mass of (2.5±0.5)kg,aged 6 to 8 months.METHODS :This experiment was carried out at the experimental animal center of Shenzhen people's Hospital between May and August 1999. 1.5cm bone segment (including periosteum)was cut off in the middle of forearm radius in 20 common grade pure NewZealand rabbits,the broken ends were covered with bone wax, 10 weeks later, the bone nonunion status was assessed by macropathological observation, pathohistological and X-ray examination.MAIN OUTCOME MEASURES:Observations on rabbit forearm radius defects by macropathological observation, pathohistological and X-ray examination.RESULTS :Twenty rabbits(40 side radius)were enrolled in this study and weeks later, bone defect region was found filled with fibrous cicatricial tissue without osseous connection ,bone wax was not absorbed, capitulum was ossified with medullary cavity blocked,a small amount of callus formed at both broken ends of fractural bone ,length of bone defect ranged from 0.8 to blocked under optical microscope,chondrocyte and osteocyte could be observed arranging disorderly and covered with fibrous membrane,defect reosseous connection could be detected at defect region at week 10,broken end was ossified and medullary cavity was blocked ,there was small amount of callus appeared at both broken ends displaying irregular shape.CONCLUSION:Bone nonunion experimental animal was successfully established on rabbits in this study, with pathological changes meeting the need of bone nonunion and displaying typical properties,which can be used as reliable and feasible experimental animal model.