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日本血吸虫肝门型童虫蛋白质SDS-聚丙烯酰胺凝胶电泳的分析
引用本文:蒋明森,杨孟祥,周述龙,李瑛.日本血吸虫肝门型童虫蛋白质SDS-聚丙烯酰胺凝胶电泳的分析[J].武汉大学学报(医学版),1995(3).
作者姓名:蒋明森  杨孟祥  周述龙  李瑛
作者单位:Department of Parasitology,Hubei Medical University,Wuhan 430071,China
摘    要:本文采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对日本血吸虫肝门型童虫蛋白组分进行了分析,结果显示:14d肝门型雌雄童虫出现33条蛋白区带,主带8条;21d肝门型雌雄童虫出现34条蛋白区带,主带8条;21d雄性童虫出现31条蛋白区带,主带7条。各组童虫与成虫比较,有27~28茶共同蛋白区带,提示日本血吸虫不同时期的肝门型童虫与成虫的蛋白组分在质与量方面虽然存在着某些差异,但总体上两者的蛋白组分有很大的相似性。

关 键 词:日本血吸虫  幼虫  蛋白质/分析  电泳.聚丙酰凝胶

Analysis of Proteins of Schistosoma Japonicum Hepato-Portal Schistosomulum by SDS-PAGE
Jiang Mingsen, Yang Mengxiang, Zhou Shulong, et al.Analysis of Proteins of Schistosoma Japonicum Hepato-Portal Schistosomulum by SDS-PAGE[J].Medical Journal of Wuhan University,1995(3).
Authors:Jiang Mingsen  Yang Mengxiang  Zhou Shulong  
Abstract:This report analyses the protein components of Schistosoma japonicum hepato-portal schistosomulum by SDS-PAGE.The experimental result shows that 14 d , 21 d,(famale and male)and 216(male) hepato-protal schistosomulum appear respectively in 33,34 and 31 protein bands,and in 8, 8 and 7 major bands protein,There are 27~28 common bands between the schistosomulum and the adult. While hepato-portal schistosomulum of different growth stages and the adult in the quality and the quantity of the protein components are somewbat different, generally they are similar in the protein components.
Keywords:schitosoma japonicum  larva  proteins/AN  electrophoresis polyacrylamide gel  
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