碱性成纤维细胞生长因子对非贴壁骨髓基质前体细胞增殖及分化作用的研究

目的　探讨碱性成纤维细胞生长因子 (bFGF)对非贴壁骨髓前体细胞的增殖及分化作用。方法　分离骨髓前体细胞体外培养 ,用碱性磷酸酶化学染色及图像分析仪测定细胞克隆数 ;采用免疫组化 (ABC法 )检测PCNA、Ⅰ型胶原、钙及骨钙素。研究bFGF对非贴壁前体细胞的促增殖及分化作用。结果　骨髓细胞中许多基质前体细胞以非贴壁前体细胞 (NASP)形式存在 ,bFGF不仅能促进其增殖 ,而且能诱导其向成骨细胞分化。结论　bFGF主要作用于非贴壁生长的基质前体细胞 :能增加骨细胞的数量 ,促进骨样组织形成 ,为临床用bFGF治疗骨折、骨不连提供理论依据。

Effect of basic fibroblast growth factor on the proliferation and differentiation of non-adherent stromal precursor cells

AIM To investigate the actions of basic fibroblast growth factor (bFGF) on the proliferation and differentiation of the non-adherent stromal precursor (NASP) cells. METHODS The osteogenic potential of the bone marrow stromal cells (BMSC) isolated from Wistar rats were cultured in the absence or presence of bFGF. After ALP cytochemistry, the colonies were counted by image analysis. The cells cultured in petri dishes were stained by the avidin-biotin-peroxidase complex (ABC) immunoperoxidase technique for collagen type Ⅰ, proliferating cell nuclear antigen ( PCNA ) and double staining for calcium and osteocalcin. RESULTS Many stromal precursor cells are present in bone marrow in a non-adherent form. bFGF not only stimulated the proliferation of NASP cells, but also enhanced the differentiation of NASP cells into osteoblasts. CONCLUSIONS NASP cells are possibly the main targets of the anabolic action of bFGF which may play an important role in fracture healing and bone formation.