| 美洛昔康对人肝癌HepG_2细胞增殖的抑制作用 |
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| 引用本文： | 李攀,赵春景.美洛昔康对人肝癌HepG_2细胞增殖的抑制作用[J].中国药房,2007,18(10):744-746. |
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| 作者姓名： | 李攀 赵春景 |
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| 作者单位： | 1. 重庆医科大学超声影像学研究所,重庆市,400010
2. 重庆医科大学附属第二医院药剂科,重庆市,400010 |
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| 摘 要： | 目的:研究环氧合酶(COX)-2抑制剂美洛昔康对肝癌细胞HepG2的生长抑制作用及其作用机制。方法:MTT法观察美洛昔康在不同浓度和作用时间下对细胞增殖的抑制作用;免疫细胞化学染色检测细胞增殖核抗原(PCNA)的表达;DNA原位末端标记(TUNEL)法和流式细胞术(FCM)检测细胞凋亡。结果:美洛昔康可抑制人肝癌细胞HepG2增殖;TUNEL及FCM检测结果均显示美洛昔康可诱导HepG2细胞凋亡。结论:美洛昔康可通过抑制细胞增殖和诱导凋亡2种途径抑制人肝癌细胞HepG2的生长。
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| 关 键 词： | 美洛昔康 COX-2 肝癌 HepG2细胞 增殖 凋亡 |
| 文章编号： | 1001-0408(2007)10-0744-03 |
| 收稿时间： | 09 1 2006 12:00AM |
| 修稿时间： | 11 16 2006 12:00AM |
Inhibitory Effects of Meloxicam on the Growth of Human Liver Cancer Cell Line HepG2 |
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| LI Pan,ZHAO Chunjing.Inhibitory Effects of Meloxicam on the Growth of Human Liver Cancer Cell Line HepG2[J].China Pharmacy,2007,18(10):744-746. |
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| Authors: | LI Pan ZHAO Chunjing |
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| Affiliation: | Institute of Ultrasound Engineering in Medicine, Chongqing University of Medical Sciences, Chongqing 400010, China;The Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China |
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| Abstract: | OBJECTIVE:To study the inhibitory effects of a COX-2 inhibitor meloxicam on the growth of human liver cancer cell line HepG2 and potential mechanism.METHODS:The effect of meloxicam on the growth of HepG2 cells was evaluated by MTT assay.The expression of proliferating cell nuclear antigen(PCNA)was assessed by immunocytochemistry method.Apotosis index(AI)was counted by terminal deoxynucleotidly transferase-mediated UTP nick end-labelling(TUNEL).The apoptosis rate(AR)was quantified by flow cytometry(FCM).RESULTS:(1)Meloxicam inhibited the proliferation of HepG2 cells.(2)The results of TUNEL and FCM confirmed that meloxicam could induce the apoptosis of HepG2 cells.CONCLUSIONS:Meloxicam suppresses the growth of HepG2 cells by inhibiting proliferation and inducing apoptosis. |
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| Keywords: | COX-2 |
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