| 铅污染对牙鲆GSH-PX酶活性的影响 |
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| 引用本文： | 王凡,赵元凤,吕景才,刘长发.铅污染对牙鲆GSH-PX酶活性的影响[J].海洋水产研究,2008,29(1):20-23. |
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| 作者姓名： | 王凡 赵元凤 吕景才 刘长发 |
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| 作者单位： | 1. 洛阳师范学院生命科学系,471022
2. 农业部海洋水产增养殖学与生物技术重点开放实验室,大连,116023;大连水产学院生命科学与技术学院,116023 |
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| 基金项目： | 国家自然科学基金项目(30271029)资助 |
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| 摘 要： | 将牙鲆置于浓度为0.5mg/L的Pb溶液中18d,测定其鳃、肌肉、全部内脏中谷胱甘肽过氧化氢酶(GSH-PX)的活性。结果表明,最初8d,各组织的GSH-PX的酶活性表现为抑制,随后各组织GSH-PX的酶活性表现为诱导;10d时,诱导幅度最大,而后表现为抑制。紧接着放入清洁海水,各组织的酶活性又恢复到对照组的水平。结果提示,对于低浓度铅的长期暴露,GSH-PX能明显指示牙鲆受海洋铅的早期污染。
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| 关 键 词： | 铅污染 牙鲆 谷胱甘肽过氧化物酶 |
| 文章编号： | 1000-7075(2008)01-0020-04 |
| 修稿时间： | 2006年11月28 |
Effect of Pb on activities of GSH-PX enzyme in tissues of Paralichthys olivaceus |
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| WANG Fan,ZHAO Yuan-feng,L Jing-cai,LIU Chang-fa.Effect of Pb on activities of GSH-PX enzyme in tissues of Paralichthys olivaceus[J].Marine Fisheries Research,2008,29(1):20-23. |
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| Authors: | WANG Fan ZHAO Yuan-feng L Jing-cai LIU Chang-fa |
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| Affiliation: | WANG Fan,ZHAO Yuan-feng,L(U) Jing-cai,LIU Chang-fa |
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| Abstract: | The effect of long term exposure to Pb(0.5mg/L) on activities of protecting enzyme GSH-PX in tissues(gill,muscle and visceral organs) of Paralichthys olivaceus was studied.It was shown that the activities of protecting enzyme GSH-PX in all tissues of P.olivaceus was inhibited on the 8th day,and was induced on the following days;on the 10th day,enzyme activity induction was at the highest level,and was inhibited again thereafter.Then after a few days' cultivation in clean water,activity of protecting enzyme GSH-PX in all tissues was reduced to the initial level.These results also suggested that GSH-PX could be used as a bio-indicator of low concentration Pb2 exposure in aquatic organisms. |
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| Keywords: | Pb Paralichthys olivaceus GSH-PX |
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