尿液中MMP-2、MMP-9及MMP-9/NGAL复合物的表达在乳腺癌筛查中的意义

目的检测女性尿液中MMP-2、MMP-9及MMP-9/NGAL复合物的表达,探讨其在乳腺癌高危发病妇女普查筛选中的意义。方法采用明胶酶底物电泳法和Westernblot方法,检测正常女性(n=60)、乳腺良性疾病患者(n=41)、乳腺癌患者(n=127)尿液中MMP-2、MMP-9及MMP-9/NGAL复合物的表达情况。结果MMP-2、MMP-9、MMP-9/NGAL复合物的表达在正常女性尿液中分别为23.33%、15%和13.33%;在乳腺良性疾病患者尿液中分别为43.93%、39.02%和36.59%;在乳腺癌患者尿液中分别为64.56%、76.37%和70.08%。乳腺癌患者尿液中MMP-2、MMP-9和MMP-9/NGAL复合物的表达与正常女性间差异有统计学意义(P<0.001);与乳腺良性疾病患者比较差异也有统计学意义(P<0.01)。乳腺癌患者尿液中MMP-2+MMP-9+MMP-9/NGAL复合物同时表达,与乳腺良性疾病患者比较差异亦有统计学意义(P<0.01)。结论1)乳腺癌、乳腺良性疾病和正常女性尿液中MMP-2、MMP-9、MMP-9/NGAL复合物均有表达。但正常女性很少表达MMP-2和MMP-9,极少表达MMP-9/NGAL复合物。2)乳腺癌患者尿液中MMP-2+MMP-9+MMP-9/NGAL复合物的同时表达,对乳腺癌的诊断具有重要意义。对乳腺癌高危发病妇女的普查筛选也具有指导意义。

The Clinical Significance of Determining Expressions of Urinary MMP-2,MMP-9 and MMP-9/NGAL Complex for Screening Patients with Breast Cancer in High-risk Female Population

Objective To investigate the potential of urinary MMP-2,MMP-9 and MMP-9/NGAL complex as biomakers for screening patients with breast cancer in high-risk female population.Methods Urinary MMP-2,MMP-9 and MMP-9/NGAL complex were detected using substrate zymography gel electrophore and their molecular identity.Urine samples were collected from healthy women(n=60),patients with benign breast disease(n=41) and patients with breast cancer(n=127).Results The expressions of MMP-2,MMP-9 and MMP-9/NGAL complex in uri...