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| 壳聚糖及其衍生物的抗氧化作用 | |
| 引用本文： | 金黎明,郑奕,杨艳,赵小菁,田文杰,王雅玲,范圣第.壳聚糖及其衍生物的抗氧化作用[J].食品与发酵工业,2008,34(11). |
| 作者姓名： | 金黎明 郑奕 杨艳 赵小菁 田文杰 王雅玲 范圣第 |
| 作者单位： | 1. 大连民族学院生命科学学院,辽宁,大连,116600 2. 天津理工大学化学化工学院,天津,300191 3. 中国海洋大学海洋生命学院,山东,青岛,266003 |
| 基金项目： | 国家自然科学基金项目 ， 辽宁省教育厅高校科研计划项目 ， 大连民族学院博士科研启动基金资助项目 |
| 摘 要： | 采用邻二氮菲-Fe2+氧化法和DPPH法,研究5种壳聚糖及其衍生物——壳聚糖(chitosan)、壳寡糖(ol-igo-chitosan)、羧甲基壳聚糖(CM-chitosan)、N-乙酰氨基葡萄糖(GLcNAc)、氨基葡萄糖盐酸盐(GlcNH2.HCl)的体外清除羟自由基和DPPH.自由基的能力。结果表明:在实验设置的浓度范围内,对羟自由基的清除能力依次为oligo-chitosan>chitosan>GLcNAc>GlcNH2.HCl>CM-Chitosan,且其清除能力随着浓度的增加而增加。其中2 mg/mL的Oligo-chitosan对羟自由基的清除率最大达到97.81%,相当于4 mmol/L的VC对羟自由基的清除能力。对DPPH.的清除能力大小依次为oligo-chitosan>chitosan>GlcNH2.HCl≥GLcNAc>CM-chitosan,且其清除能力随着浓度的增加而增加。2 mg/mL oligo-chitosan的清除率为97.34%,大于6mmol/L VC的清除速率79.92%。2种方法测定的结果比较一致。 |
| 关 键 词： | 壳聚糖 衍生物 抗氧化性 1 1-二苯代苦味酰基自由基(DPPH·) 羟自由基 |
Study on Antioxidant Activity of Chitosan and Its Derivatives |
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| Jin Liming,Zheng Yi,Yang Yan,Zhao Xiaojing,Tian Wenjie,Wang Yaling,Fan Shengdi.Study on Antioxidant Activity of Chitosan and Its Derivatives[J].Food and Fermentation Industries,2008,34(11). | |
| Authors: | Jin Liming Zheng Yi Yang Yan Zhao Xiaojing Tian Wenjie Wang Yaling Fan Shengdi |
| Affiliation: | Jin Liming1,Zheng Yi2,Yang Yan3,Zhao Xiaojing1,Tian Wenjie1,Wang Yaling1,Fan Shengdi11 |
| Abstract: | The antioxidant activities in vitro of chitosan,caboxymethyl-chitosan(CM-Chitosan),oligo-chitosan,N-acetyl-glucosamine(GLcNAc) and GlcNH2·HCL were investigated by phenanthroline-Fe2+ and DPPH methods.The results showed that in the setting concentration range,the antioxidant activities increased with the elevation of concentration.The activity of scavenging hydroxyl free radical of chitosan and its derivatives was that Oligo-chitosan >Chitosan>GLcNAc >GlcNH2·HCL>CM-Chitosan.The highest scavenging rate of hydroxyl free radical using 2 mg/mL of Oligo-chitosan could be 97.81%,which was similar to the activity using 4 mmol/L of VC.The activity of scavenging DPPH free radical was that Oligo-Chitosan>Chitosan>GlcNH2·HCL≥GLcNAc>CM-Chitosan.The highest scavenging rate of DPPH free radical using 2 mg/mL Oligo-chitosan could be 97.34%,more than the rate 79.92% of 6mmol/L VC.The results were consistent by two methods. |
| Keywords: | chitosan derivatives antioxidant activity 1 1-diphenyl-2-pierylhydrazy(DPPH·) hydroxyl free radical |
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