离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素

采用离子色谱（IC）和光化学衍生荧光检测（PCF）联用技术，建立了保健品中核黄素的分析方法.色谱柱选用Dionex Ion Pac AG11-HC保护柱(4 mm×50 mm)和Ion Pac AS11-HC分析柱(4 mm×250 mm)，淋洗液为40 mmol·L^-1 NaOH. 待测物通过自制光化学反应器（254 nm）在线衍生，核黄素转化为强荧光物质，从而以荧光检测器检测.在优化的实验条件下，对50 mg·L^-1的核黄素溶液平行测定6次的保留时间和峰面积相对标准偏差分别为0.6%和2.1%（n=6）.核黄素的浓度在10~100 mg·L^-1范围内与峰面积呈现良好的线性关系，线性相关系数r =0.993，检出限（S/N=10）为0.5 mg·L^-1，回收率为（101.46±2.51）%.

Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection

Ion chromatography method（IC） in conjunction with photochemical fluorimetry （PCF） was developed to determine riboflavin in health protection products. Chromatographic separation of the treated sample was performed on Dionex IonPac AGll-HC（4 mm× 50 mm） guard column and IonPac ASll-HC（4 mm× 250 mm） analytical column with 40 mmol L-1 NaOH as eluent. The column effluents were subjected on-line to homemade UV irradiation（254 nm）reac- tor, riboflavin was transformed to a strongly fluorescent compound and detected by spectrofluorimetor. Under the optimum conditions, the relative standard deviations（RSDs） of retention time and peak area of 50 mg L-1 riboflavin were 0.6% and 2.1% （n = 6）. The concentration of riboflavin showed good linear relationship with peak area in range of 10 - 100 mg L-1 with r= 0. 993, the limit of detection（S/N= 10）was 0. 5 mg L-1 and the recovery was （101.46 ± 2.51） %.