Sel1L 对小鼠骨髓源树突状细胞的影响 |
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引用本文: | 许洁,颜楠楠,赵传祥,李慈,吴仪,肖腾飞,高凤威,周文慧,邵启祥,龙乔明,夏圣. Sel1L 对小鼠骨髓源树突状细胞的影响[J]. 中国免疫学杂志, 2018, 34(5): 727 |
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作者姓名: | 许洁 颜楠楠 赵传祥 李慈 吴仪 肖腾飞 高凤威 周文慧 邵启祥 龙乔明 夏圣 |
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作者单位: | 江苏大学医学院免疫学与免疫检验学教研室;江苏大学检验医学研究所;苏州大学实验动物中心 |
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摘 要: | 目的:研究Sel1L(Suppressor/ enhancer of Lin-12-like)基因对小鼠骨髓源树突状细胞(BMDCs)分化及其功能的影响。方法:利用Cre-Loxp 重组系统构建Sel1L 基因敲除小鼠模型,用ELISA 和实时荧光定量法检测BMDCs 分泌细胞因子IL-6、IL-12 的表达;用免疫印迹法(Western bolt)检测BMDCs 细胞中Sel1L蛋白的表达;用流式细胞术检测BMDCs 细胞CFSE、细胞表面分子CD80、CD86、MHC-Ⅰ、MHC-Ⅱ及其对特异性CD4+ T 细胞抗原提呈能力的影响。结果:Sel1L的缺失抑制BMDC诱导分化过程中的增殖效率,上调DCs 分泌因子的能力和MHC-Ⅰ的表达,减少MHC-Ⅱ的表达,并抑制BMDCs 细胞对OVA323-339 抗原特异性T 细胞的增殖。结论:Sel1L 缺失可以抑制小鼠骨髓源树突状细胞的分化,下调DC 对OVA 特异性的CD4+ T细胞的抗原提呈功能。
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关 键 词: | Sel1L 树突状细胞 内质网应激 共刺激分子 CD4+ T 细胞 |
Effects of Sel1L on properties of bone marrow derived dendritic cells |
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Abstract: | Objective:To explore the effects of suppress or enhancer of lin-12-like(Sel1L) on differentiation and function of bone marrow-derived dendritic cells.Methods:To generate conditional knockout mice by the Cre-Loxp recombination system.ELISA and Real-time fluorescence quantitative PCR(RT-PCR) was used for analyzing the protein levels and mRNA levels of IL-6/IL-12 in BMDCs.The protein levels of Sel1L in BMDCs were detected by Western bolt.The expression of CFSE,CD80,CD86,MHC-Ⅰ,MHC-Ⅱon BMDCs and the capability in priming OVA specific CD4+ T cells proliferation were analyzed by the flow cytometry.Results:The deficiency of Sel1L decreases the proliferation of DCs during its differentiation,up-regulates the secretion of IL-6,IL-12 and the expression of MHC-Ⅰ.Notably,Sel1L-null DCs was failed to up-regulate MHC-Ⅱexpression and dramatically impaired their ability to prime OVA323-339 specific CD4+ T cell.Conclusion:The deletion of Sel1L can reduce the proliferation of BMDCs and down-regulate its ability in priming the proliferation of OVA specific CD4+ T cells. |
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