HPLC法同时测定5种常见中药材中齐墩果酸和熊果酸的含量

目的:建立测定含有齐墩果酸与熊果酸中药材的方法,并测定这些中药材中齐墩果酸与熊果酸的含量,为其品质评价提供依据。方法:采用高效液相色谱法。色谱柱为Welch Materials Ultimate XB C18(250mm×4.6mm,5μm),检测波长为210nm,流动相为甲醇-0.1%磷酸-三乙胺(86∶14∶0.05,V/V/V),流速为0.6mL·min-1。结果:齐墩果酸进样量在0.330～3.300μg范围内与峰面积积分值呈良好线性关系(r=0.9998),平均加样回收率为98.26%,RSD=0.47%(n=6);熊果酸进样量在0.390～3.900μg范围内与峰面积积分值呈良好线性关系(r=0.9994),平均加样回收率为97.98%,RSD=0.50%(n=6)。结论:本方法简便、准确、重复性好,可为含有齐墩果酸与熊果酸中药材的质量控制提供方法参考,且本试验数据可为这些中药材的质量评价提供科学依据。

Simultaneous Determination of Oleanolic Acid and Ursolic Acid in 5 Common Chinese Medicinal Materials by HPLC

OBJECTIVE： To establish a method for the simultaneous determination of oleanolic acid and ursolic acid in Chinese medicinal material, and to provide scientific basis for quality evaluation. METHODS： HPLC method was adopted. Welch Materials Ultimate XB C I8（250 mm×4.6 mm, 5μm） column was used with the mobile phase consisted of methanol-0.1% phosphoric acid-tri- ethylamine（86 ： 14：0.05） at a flow rate of 0.6 mL·mL^-1. The detection wavelength was set at 210 nm. RESULTS： The linear rang- es of oleanolic acid and ursolic acid were 0.330-3.300μg （r=0.999 8） and 0.390-3.900 μg （r=0.999 4）. Average recoveries were 98.26% （RSD=0.47% ,n=6） and 97.98%（RSD=0.50% ,n=6）. CONCLUSION： The method is simple, accurate, reproduc- ible, and suitable for the quality control and quality evaluation of Chinese medicinal materials containing ursolic acid and oleanolic acid.