芦荟多糖联合茶多酚对小鼠酒精性肝损伤的预防作用

目的:研究芦荟多糖(AG)联合茶多酚(TP)对酒精性肝损伤的预防作用。方法:以芦荟凝胶粉为原料,提取芦荟多糖;以C57BL/6小鼠为动物模型,4周42% vol酒精加1次50% vol酒精灌胃,造成酒精性肝损伤,检测小鼠肝脏指数、血清生化指标、肝脏生化指标和病理变化,比较AG、TP和AG&TP对酒精性肝损伤的预防作用。结果:模型组与空白组相比,肝脏指数、血清总胆固醇(TC)、甘油三酯(TG)、谷丙转氨酶(ALT)和谷草转氨酶(AST)活性极显著升高;还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性极显著性降低(P<0.01),而丙二醛(MDA)极显著增加(P<0.01);肿瘤坏死因子(TNF-α)以及白介素-6(IL-6)水平也极显著增高(P<0.01),HE染色结果显示肝细胞明显的炎性浸润和空泡变性。AG&TP组与AG和TP组相比,AG&TP可以更加显著(P<0.05)地改善酒精引起的氧化损伤、炎症反应和肝形态学变化。结论:芦荟多糖联合茶多酚能够有效缓解酒精性肝损伤,且效果优于单一成分。

Preventive Effect of Aloe Polysaccharide Combined with Tea Polyphenol on Alcoholic Liver Injury in Mice

Objective:This study was aim to investigate the preventive and therapeutic effects of aloe polysaccharide(AG)combined with tea polyphenol(TP)on alcoholic liver injury. Methods:Aloe polysaccharide was extracted from aloe gel powder. C57BL/6 mice were used as animal model,and the mice were fed with 42%vol alcohol for 4 weeks and a high dose of 50%vol alcohol for 1 days,which induced the alcoholic liver injury. In addition,the liver index and serum biochemical index,liver biochemical index and pathological changes in mice were measured. Finally,the effect of AG,TP and AG combined TP were compared. Results:Compared with the control group,the liver index,serum total cholesterol(TC),triglyceride(TG),alanine aminotransferase(ALT)and alanine aminotransferase(AST)activities in the model group were significantly higher than those in the control group. The activity of glutathione(GSH),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)were decreased significantly while malondialdehyde(MDA)increased significantly. The levels of tumor necrosis factor α(TNF-α)and interleukin-6(IL-6)were also significantly increased,and HE staining showed obvious inflammatory infiltration and vacuolar degeneration of hepatocytes. AG&TP could significantly(P<0.05)improve the oxidative injury,inflammatory response and liver morphological changes induced by alcohol compared with AG or TP. Conclusion:Aloe polysaccharide combined with tea polyphenol could effectively alleviate alcoholic liver injury and its effect was more significant than single component.