蛋白芯片检测丙型肝炎病毒分片段抗体

目的用蛋白芯片的方法检测丙型肝炎病毒(HCV)分片段抗体。方法利用生物芯片技术和化学发光技术将高度纯化的抗原HCV Core、NS3、NS4、NS5以特定微阵列固定在固相载体上,用化学发光免疫法检测抗HCV Core、抗HCV NS3、抗HCV NS4和抗HCV NS5。结果174份血清中HCV抗体阳性87份,HCV抗体阴性87份,其中献血员33份,非丙型肝炎的其他肝病患者39份,非肝病者15份。87例雅培公司的AxSYM(微粒子发光)检测抗HCV阳性患者中,蛋白芯片检出阳性85份,阳性率为97.70%;在174份标本中,蛋白芯片检出阳性标本88份,其中抗HCV阳性87份,阳性率98.86%;抗HCV NS3检出49份,阳性率为55.68%;抗HCV NS4检出68份,阳性率为77.27%;抗HCV NS5检出37份,阳性率为42.05%;抗HCV Core检出69份,阳性率为78.41%。结论蛋白芯片显示较高的敏感性和特异性,具有临床实用价值。

Detection of anti hepatitis C virus antibodies to different antigenic epitopes with protein chip

Objective To detect antibodies against different antigenic epitopes of hepatitis C virus(HCV) with protein chip. Methods Highly purified HCV Core,NS3,NS4,NS5 proteins were immobilized on solid matrix by biochip arrayer,the protein chip was used to detect the corresponding antibodies in human serum by chemiluminescent immunoassays. Results 87 anti-HCV positive and 87 anti-HCV negative serum samples were tested by the protein chip and Abbott AxSYM anti-HCV(microparticle enzyme immunoassay) respectively in order to compare the two methods.Among the 87 serum samples that were detected antiHCV positive by the Abbott AxSYM assay,85 were anti-HCV positive by the protein chip,with the positive rate of 97.7%.Among the 174 serum samples,88 cases showed positive results by the protein chip,87 cases were anti-HCV positive(98.6%),49 cases were anti-NS3 positive(55.68%),68 cases were anti-NS4 positive(77.27%),37 case were anti-NS5 positive(42.05%),and 69 cases were anti-Core positive(78.41%). Conclusions Protein chip showed relative high sensitivity and specificity.The protein chip was an efficient technique and might be used clinically.