| 一株木质纤维素酶高产菌株的选育及产酶条件研究 |
| |
| 引用本文： | 陈英连, 付正, 张良雨, 管斌, 孔青, . 一株木质纤维素酶高产菌株的选育及产酶条件研究[J]. 食品工业科技, 2014, (18): 189-193. DOI: 10.13386/j.issn1002-0306.2014.18.032 |
| |
| 作者姓名： | 陈英连 付正 张良雨 管斌 孔青 |
| |
| 作者单位： | 中国海洋大学食品科学与工程学院;山东医学高等专科学校 |
| |
| 基金项目： | “十二五”农村领域国家科技计划(2013BAD10B02-06);青岛市公共领域科技支撑计划项目(11-2-3-63-nsh);啤酒生物发酵工程国家重点实验室开放课题(K2012002) |
| |
| 摘 要： | 主要通过紫外(UV)和甲基磺酸乙酯(EMS)的交替诱变的方法提高绿色木霉的产酶能力,并成功筛选到了一株产酶活性较高的菌株,其在平板筛选培养基中的透明圈直径与菌落直径之比达到2.1,传代稳定后在产酶培养基中纤维素酶活(CMCase)和滤纸酶活(FDAase)分别达到21.05U/mL和2.4U/mL。之后经培养基的优化进一步提高其酶活,最终诱变菌株在最佳产酶培养基中CMCase达到22.5U/mL,FDAase达到2.52U/mL。
|
| 关 键 词： | 绿色木霉 诱变 纤维素酶 液体发酵 |
| 收稿时间： | 2013-12-20 |
The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production |
| |
| CHEN Ying-lian, FU Zheng, ZHANG Liang-yu, GUAN Bin, KONG Qing, . The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production[J]. Science and Technology of Food Industry, 2014, (18): 189-193. DOI: 10.13386/j.issn1002-0306.2014.18.032 |
| |
| Authors: | CHEN Ying-lian FU Zheng ZHANG Liang-yu GUAN Bin KONG Qing |
| |
| Affiliation: | CHEN Ying-lian;FU Zheng;ZHANG Liang-yu;GUAN Bin;KONG Qing;Food Science And Engineering College,Ocean University of China;Shandong Medical College; |
| |
| Abstract: | The cellulase production strain T. viride 8140 was treated with UV and EMS. The mutant strain T.viride8140UEUE4-80 was showed to have a higher cellulase activity than others and the Hc of the mutant reached 2.1. After several passages its CMCase and FDAase were stabilized at 21.05U/mL and 2.4U/mL. The conditions of fermentation were studied and the optimal culture medium was decided. Under the optimal culture medium, the CMCase and FPAase of the mutant separately reached 22.5, 2.52U/mL. |
| |
| Keywords: | T.viride mutagenesis cellulose liquid fermentation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《食品工业科技》浏览原始摘要信息 |
|
点击此处可从《食品工业科技》下载全文 |
