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| 43份茶树品种资源遗传多样性的SSR研究 | |
| 引用本文： | 谭月萍,李娟,刘硕谦,晏嫦妤,陈金华.43份茶树品种资源遗传多样性的SSR研究[J].茶叶科学,2009,29(4). |
| 作者姓名： | 谭月萍 李娟 刘硕谦 晏嫦妤 陈金华 |
| 作者单位： | 1. 湖南省茶业有限公司,湖南,长沙,410002 2. 湖南农业大学茶学教育部重点实验室,湖南,长沙,410128 3. 广东省农业科学院茶叶研究所,湖南,广州,510640 |
| 基金项目： | 湖南农业大学科学基金项目(编号:07WD22)资助 |
| 摘 要： | 采用SSR分子标记技术对43个茶树品种(系)进行了遗传多态性分析。用37对可扩增引物对43个茶树品种(系)扩增,经8%非变性聚丙烯酰胺凝胶电泳检测,其中有34对引物产生多态性,不同引物扩增带数分布在1～11条之间,扩增片段大小介于150～350bp。应用DPS软件,进行遗传距离和相似性系数计算,43份材料遗传距离的变化范围在0.059～0.820之间,说明供试茶树资源间的遗传变异十分宽广。根据UPGMA法构建聚类树状图,在平均遗传距离水平上,可将43份材料划分为7个类群。 |
| 关 键 词： | 茶树 SSR分子标记 遗传多样性 |
Genetic Diversity of 43 Tea Cultivars (Camellia sinensis(L.) O.Kuntze) by SSR Markers |
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| TAN Yue-ping,LI Juan,LIU Shuo-qian,YAN Chang-yu,CHEN Jin-hua.Genetic Diversity of 43 Tea Cultivars (Camellia sinensis(L.) O.Kuntze) by SSR Markers[J].Journal of Tea Science,2009,29(4). | |
| Authors: | TAN Yue-ping LI Juan LIU Shuo-qian YAN Chang-yu CHEN Jin-hua |
| Affiliation: | 1. Key Lab of Tea Science of Ministry of Education;Hunan Agricultural University;Changsha 410128;China;2. Hunan Tea Company;Ltd.;Changsha 410002;3. Tea Research Institute;Guangdong Academy of Agricultural Sciences;Guangzhou 510640;China |
| Abstract: | SSR molecular marker were used to analyze the genetic polymorphism of tea germplasm.These 37 pairs of primer were amplified with 43 tea cultivars,and their PCR products were visualized by 8% native polyacrylamide gel electrophoresis. The polymorphic alleles were visualized from the PCR products amplified by 34 pairs of primer out of 37. The number of bands per primer pair ranged from 1 to 11 and the SSR fragment size of different SSR locus ranged from 150 to 350 bp. Based on the SSR results,the genetic dist... |
| Keywords: | tea plant [Camellia sinensis (L ) O Kuntze] SSR genetic diversity |
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