Protective activity of Hertia cheirifolia extracts against DNA damage,lipid peroxidation and protein oxidation |
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Authors: | Seoussen Kada Abderrahmane Senator Ibrahim Demirtaş Tevfik Özen Bircan Çeken Toptanci |
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Affiliation: | 1. Laboratory of Applied Biochemistry, Faculty of SNV, University Ferhat Abbas, Sétif, Algeria;2. Department of Chemistry, Faculty of Science, Cank?r? Karatekin University, Cank?r?, Turkey;3. Department of Chemistry, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey;4. Department of Chemistry, Faculty of Science and Arts, University of Dicle, Diyarbak?r, Turkey |
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Abstract: | Context: Hertia cheirifolia L. (Asteraceae), a perennial shrub widely distributed in Northern Africa, is traditionally used to treat inflammatory disorders.Objective: The protective effect of methanol (Met E) and aqueous (Aq E) extracts of Hertia cheirifolia against DNA, lipid and protein oxidation was investigated.Materials and methods: Different concentrations (50–1000?μg/mL) of Hertia cheirifolia aerial part extracts were examined against DNA, lipid and protein oxidation induced by H2O2?+?UV, FeSO4, and Fe3+/H2O2-ascorbic acid, respectively. The DPPH?, metal ion chelating, reducing power and β-carotene bleaching tests were conducted.Results: Both extracts were rich in polyphenols, flavonoids and tannins, and were able to scavenge DPPH? with IC50 values of 138 and 197?μg/mL, respectively. At 300?μg/mL, Aq E exerted stronger chelating effect (99%) than Met E (69%). However, Met E reducing power (IC50?=?61?μg/mL) was more than that of Aq E (IC50?=?193?μg/mL). Both extracts protected from β-carotene bleaching by 74% and 94%, respectively, and inhibited linoleic acid peroxidation. The inhibitory activity of Aq E extract (64%) was twice more than that of Met E (32%). Interestingly, both extracts protected DNA against the cleavage by about 96–98%. At 1?mg/mL, Met E and Aq E restored protein band intensity by 94–99%.Conclusions: Hertia cheirifolia exhibits potent antioxidant activity and protects biomolecules against oxidative damage; hence, it may serve as potential source of natural antioxidant for pharmaceutical applications and food preservation. This is the first report on the protective activity of this plant against biomolecule oxidation. |
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Keywords: | Antioxidant activity biomolecule lesions medicinal plant oxidative stress polyphenols |
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