幽门螺杆菌感染与胃癌侵袭转移及MMP-2，TIMP-2表达的关系

目的：探讨H.pylori感染与胃癌侵袭转移及MMP-2，TIMP-2表达的关系。方法：采用Warthin-starry组织染色法、快速尿素酶试验及14C-UBT检测H.pylori感染，免疫组织化学法检测22例H.pylori感染胃癌与22例无H.pylori感染胃癌组织MMP-2，TIMP-2表达。结果：H.pylori感染胃癌组浆膜侵袭率与淋巴结转移率分别为81.8%与72.7%，高于无H.pylori感染胃癌组（P<0.05）。胃癌浆膜侵袭组、淋巴结转移组MMP-2表达阳性率分别为96.6%与100.0%，高于无浆膜侵袭组、无淋巴结转移组（分别为40.0%与50.0%）；TIMP-2表达阳性率分别为48.3%与45.8%，低于无浆膜侵袭组、无淋巴结转移组（分别为86.7%与80.0%）；MMP-2／TIMP-2值分别为6.86与7.94，高于无浆膜侵袭组、无淋巴结转移组（分别为0.87与1.07）。H.pylori感染胃癌组MMP-2表达阳性率为90.9%，高于无H.pylori感染胃癌组（63.6%）；TIMP-2表达阳性率为36.4%，低于无H.pylori感染胃癌组（P<0.01）；MMP-2/TIMP-2值为7.73，高于无H.pylori感染胃癌组（P<0.01）。结论：H.pylori感染胃癌浆膜侵袭和淋巴结转移增多，与MMP-2表达增加、TIMP-2表达减少、MMP-2/TIMP-2值升高有关。

Helicobacter pylori infection, the invasion and metastasis of the gastric carcinoma, and the expression of MMP-2 and TIMP-2

Objective To explore the relationship among Helicobacter pylori (H.pylori) infection, the invasion and metastasis of the gastric carcinoma, and the MMP-2 and TIMP-2 expression.Methods The Warthin-starry method, rapid urease test and 14C-UBT were used to detect the infection of H.pylori.The expression of MMP-2 and TIMP-2 in 22 patients with gastric carcinoma infected by H.pylori and 22 patients with gastric carcinoma but not infected were detected by immunohistochemistry method. Results The incidence of serosal invasion and lymph node metastasis in patients with gastric carcinoma infected by H.pylori ( 81.8% and 72.7% respectively) was significantly higher than that in patients with gastric carcinoma but not infected ( 50.0% and 36.4% respectively, P<0.05 ).The positive rates of MMP-2 expression in the invasive group (96.6%) and in the metastasis group (100.0%) were significantly higher than that in the non-invasive group (40.0%) and the non-metastasis group (50.0%), whereas the positive rates of TIMP-2 expression in the invasive group (48.3%) and in the metastasis group (45.8%) were significantly lower than that in the non- invasive group (86.7%) and the non-metastasis group ( 80.0%).The ratios of MMP-2/TIMP-2 in the invasive group (6.86) and in the metastasis group ( 7.94) were significantly higher than that in the non-invasive group (0.87) and the non-metastasis group (1.07).The positive rate of MMP-2 expression in patients with gastric carcinoma infected by H.pylori (90.9%) was significantly higher than that in patients with gastric carcinoma but not infected (63.6%),whereas the positive rate of TIMP-2 expression in patients with gastric carcinoma infected by H.pylori ( 36.4%) was significantly lower than that in patients with gastric carcinoma but not infected(P<0.01).The ratio of MMP-2/TIMP-2 in patients with gastric carcinoma infected by H.pylori (7.73) was significantly higher than that in patients with gastric carcinoma not infected (P<0.01). Conclusion It is associated with the increase of the MMP-2 expression, the decrease of the TIMP-2 expression and the increase of the ratio of MMP-2/TIMP-2 that the incidence of serosal invasion and lymph node metastasis in patients with gastric carcinoma increases by H.pylori infection.