HMME-PDT对大鼠致龋模型中变形链球菌抑制作用的研究

目的 探讨不同参数光动力疗法(PDT)在大鼠口腔龋齿预防中的作用.方法 用变形链球菌感染Wistar大鼠口腔,建立龋齿模型.以0.9%生理盐水、0.2%氟化钠为对照组,单纯激光、单纯光敏剂、不同参数PDT为实验组.采用血卟啉单甲醚(HMME)为光敏剂,532 nm半导体激光为光源,每周处理牙齿1次,5周后处死大鼠,Ke...

Inhibitive effect of photodynamic therapy with different parameters on caries development in rats

Objective To evaluate the inhibitive effect of Photodynamic therapy (PDT) with different parameters on dental caries formation in rats.Methods Wistar rats were infected with S.mutans to establish the rat caries mode 1.The rat's teeth were treated with 0.9% NS and 0.2% NaF as controls;laser,photosensitizer and laser plus photosensitizer,respectively as treated groups.Hematoporyrin monomethyl ether(HM ME) was used as photosensitizer and laser as the light source.The rats were sacrificed after five weeks.Keyes count was used to measure the inhibitory effect of PDT on dental caries.The surface morphology of the teeth was evaluated by scan electronic microscopy(SEM).Results The PDT-treated group and NaF-treated group showed significantly reduced Keyes scores compared with the negative controls (P＜0.05).The laser-treated group,photosensitizer-treated group and NS-treated group showed no significantly reduced Keyes scores compared with the negative controls (P＜0.05).The differences of Keyes scores between various parameter PDT treated groups were significant (P＜0.05).Additionally,Keyes scores reduced gradually with the increasing of laser power.The outcome of SEM showed that the teeth treated with different laser powers exhibited different changes in morphology.Conclusion HMMEPDT had significant inhibitive efficacy on caries development in rats and its inhibitory effect presented close correlation with the power of laser,which indicates that it is important to apply appropriate power in clinical treatment.