非小细胞肺癌组织P—ACC和NF—kB表达意义及其相关性研究

目的：探讨非小细胞肺癌（non—smallcelllungcancer，NSCLC）组织中乙酰辅酶A羧化酶磷酸化后的产物（ace—tyl—coacarboxylaseproductofphosphorylation，P-Acc）与核转录因子KB（nuclearfactorkappaB，NFKB）的表达和相关性及其与临床病理因素的关系。方法：以赣州市肿瘤医院（赣州市肿瘤防治研究所）2008-01—012010—123l有完整临床病理资料，病理检查确诊为NSCI。C的62例癌组织作为研究组，20例同期因其他原因行肺叶切除患者的正常肺组织作为对照组。免疫组化SP法检测P—ACC和N卜KB的表达情况。结果：P—ACC在正常肺组织中80．0％（16／20）阳性表达，在NSCLC组织中33．9％（21／62）阳性表达，差异有统计学意义，P=0．0001；NF-xB在正常肺组织中15．0％（3／20）阳性表达，在NSCI。C组织中75．8％（47／62）阳性表达，差异有统计学意义，P〈0．05。在NSCI。C组织中，P-ACC的阳性表达与肿瘤大小显著相关，P=0．033；而与淋巴结转移、临床分期及病理类型无关，P〉0．05。NF—xB的阳性表达与肿瘤大小、淋巴结转移及临床分期无关，P〉0．05；而与病理类型显著相关，P=0．008。P—ACC与NF_KB的阳性表达之间呈显著负相关，r=-0．409，P〈O．001。结论：P-ACC阳性表达降低可能激活NF-xB阳性表达，促进NSCLC的发生、发展及侵袭转移。

Expression and correlation of P-ACC and NF-kB in non-small cell lung cancer tissues

OBJECTIVE： To explore the expression of acetyl-coa carboxylase product of phosphorylation （P ACC） and nuclear factor kappaB（NF-kB） in non small cell lung cancer（NSCLC） tissue and the relationship of the tumor size, lymph node metastasis, clinical stage and pathological type, and study the correlation of them. METHODS： 62 cases of NSCLC tissue were as a patient group. Twenty cases normal lung tissues as controls. Immunohistochemical SP method was used in detection of the expression of P-ACC and NF-kB in lung cancer and normal lung tissue. The immunohisto chemical SP expression of P-ACC and NF-kB was investigated in 60 NSCLC lung cancer tissue and 20 lung normal tissue because of other reasons for lobectomy. RESULTS： PACC 80.0% （16/20） positive expression in normal lung tissue and 33.9%（21/62） positive expression in NSCLC tissues,the difference was significant （P=0. 000 1）. NF-eB was 15% posi- tive expression in normal lung tissue and 75.8%（47/62） positive expression in NSCLC tissues,the difference was signifi- cant （P〈0. 05）. In NSCLC, the positive expression of P-ACC was associated with tumor .size significantly （P = 0. 033）, but it was no correlation between lymph node metastasis,clinical stage and pathological type. We also found that there was no correlation between the positive expression of NF-kB and tumor size, lymph node metastasis, clinical stage but there was significant correlation with pathological type （P=0. 008）. Further analysis revealed that the positive expression of P- ACC and NF-eB in NSCLC was significantly negative correlation （r= - 0. 409, P〈0.001 ）. CONCLUSION： The reduced positive expression of P-ACC may activate positive expression of NF-kB, and promote the occurrence, development, inva- sion and metastasis of NSCLC.