宫颈癌组织ILK和MMP9表达临床意义分析

目的：探讨宫颈病变组织中整合素连接激酶（integrin—linkkinase，ILK）、基质金属蛋白酶-9（matrixmetalloprotei—nases，MMP-9）的表达及其临床意义。方法：免疫组化方法检测2010—09-01—2012-06-30我院门诊及住院的60例宫颈鳞状细胞癌、100例宫颈上皮内瘤变（cervicalintraepithelialneopla，CIN）、40例慢性宫颈炎组织中ILK和MMP-9的表达。结果：1）宫颈癌组织中ILK表达率为80．0％（48／60），明显高于正常宫颈黏膜上皮的0％（0／40），X2=61．54，P〈0．001；高于CINI～Ⅱ的30．77％（16／52），X2=27．27，P〈O．001；也高于CINlll的45．83％（22／48），X2=13．65，P〈O．001。而CINI～Ⅱ与CINHI之间ILK的表达差异无统计学意义，X2=2．404，P=0．121。宫颈癌组织中MMP-9表达率为88．33％（53／60），明显高于正常宫颈黏膜上皮（0／40），YX2=75．18，P〈0．001；高于CINI～Ⅱ的28．85％（15／52），X2=41．33，P〈O．001；但与CINⅢ的79．17％（38／48）相比差异无统计学意义，X2=1．69，P=0．194。MMP-9在CINⅢ的表达明显高于CINI～Ⅱ,X2=25．37，P〈0．001。ILK表达与临床分级、组织学分级和淋巴结转移有关，P值均〈O．05。MMP-9与淋巴结转移有关，P=0．012。ILK和MMP9在宫颈癌组织中表达呈正相关，r=0．429，P〈0．05。结论：ILK、MMP-9在宫颈癌组织中均呈过表达，且两者的过表达可能在宫颈癌浸润转移过程中起重要作用。

Expression of ILK and MMP-9 in CIN and cervical cancer tissues

OBJECTIVE：To investigate the expression of ILK and MMP-9 in different cervical lesion. METHODS： The expression of ILK and MMP-9 were detected by SP in 40 cases of chronic cervicitis, 100 cases of cervical intraepitheli- al （CIN） and 60 cases of ceivical cancer. RESULTS：The expression of ILK was higher in cervical cancer 1-80.0 % （48/60）] than that in chronic cervicitis （Xz =61.54,P〈0. 001） ,the group of CIN I-Ⅱ（2 =27.27,P〈0. 001）or CINⅢ （X2 = 13.65,P〈0. 001）. There was no significant difference between the group of CIN I-Ⅱ and the group of CIN Ⅲ （X2 = 2. 404, P= 0. 121）. The expression of MMP-9 was higher in cervical cancer 1-88.33 % （53/60）] than that in chronic cervi- citis I-0（0/40） ,X2=75.18,P〈0. 001] and the group of CINI-Ⅱ [28.85%（15/52） ,X2 =41.33,P〈0. 001],but when compared with CINIII 1-79.17 % （38/48）], there was no significant difference （z = 1.69, P= 0. 194） ; The expression of MMP-9 is higher in CINIll than that in CIN I-Ⅱ （X2 = 25.37,P〈0. 001）. The expression of ILK related with clinical stage （P=0. 008） ,lymph node metastasis （P=0. 005） ,histological grade （P=0. 004） The expression of MMP-9 related with lymph node metastasis （P=0. 012）. Positive correlation was found among the expression of ILK and MMP-9 （r= 0. 429, P〈0.05）. CONCLUSIONS.. The expression of ILK and MMP-9 was higher in cervical cancer. The high expression of ILK and MMP-9 relate with the occurence and development of cervical cancer, they may play an important role in angio- genesis of cervical squamous carcinoma.