井冈山血水草种群遗传多样性的RAPD分析

采用RAPD技术对江西省井冈山5个血水草种群进行了遗传多样性研究。从50条随机引物中筛选出16条随机引物对血水草的90个个体进行了扩增，共得到180条可统计条带，其中156条为多态带，占总条带数的85.56%。根据Shannon指数和Nei’s遗传分化指数分析显示5个种群间的遗传多样性为31.50%、遗传分化系数为0.302 9，表明血水草大部分遗传变异存在于种群内，少部分存在种群间。血水草的基因流（Nm）为1.150 8。采用算数平均数的非加权成组配对法（UPMGA）对Nei’s的一致度进行的聚类分析结果显示梨坪种群和水口种群的遗传距离最近（0.120 9），茨坪种群和荆竹山种群的遗传距离最远（0.234 9）。Mantel相关性分析结果显示血水草种群间的遗传距离不仅与海拔梯度有关，还与其生境有关。

Genetic diversity of Eomecon chionantha Populations in Jinggangshan revealed by RAPD

The genetic diversity of Eomecon chionantha Hance populations in five different habitats,namely,bamboo forest,evergreen broad-leaved forest,shrubs,conifer and broad-leaved forest and fir forest,was analyzed by polymorphic DNA technique.Sixteen primers were selected from 50 random primers amplified 180 fragments in 90 individuals of E.chionantha,in which 156 segments were polymorphic.The rate of polymorphism was 85.56%.By Shannon index,the ratio of genetic diversity among 5 populations was 31.50%,the Nei's g...