胡芦巴总皂苷的提取纯化工艺考察

目的：制备高纯度的胡芦巴总皂苷。方法：采用乙醇回流法提取胡芦巴总皂苷，依次加正己烷、三氯甲烷、乙酸乙酯、正丁醇进行系统溶剂萃取，三氯甲烷有效部位经HPD-400型大孔吸附树脂富集纯化。采用显色反应和TLC鉴别总皂苷，运用UV测定胡芦巴总皂苷含量。结果：三氯甲烷萃取部位中总皂苷纯度40%，得率6.28%；水洗脱液中胡芦巴总皂苷纯度80.5%，得率0.32%。结论：优选的提取纯化工艺快速简便，为胡芦巴总皂苷的药效学评价奠定基础。

Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen

Objective: To prepare total saponins of Trigonellae Semen with high purity. Method: Total saponins of Trigonellae Semen was extracted by ethanol refluxing method,then extract was dealt with different solvents systematically,including n-hexane,chloroform,ethyl acetate,n-buthanol.Chloroform effective part was purified through HPD-400 macroreticular resin.Total saponins in each steps and fractions was identified by chromogenic reaction and TLC,contents of total saponins were determined by ultraviolet spectroscopy. Result: Purity of total saponins in chloroform solvent extraction was 40% with yield of 6.28%;Purity of total saponins in water eluent was 80.5% with yield of 0.32%. Conclusion: This optimized process was convenient and efficient,it could lay foundation for pharmacodynamic study.