莪术醇固体脂质纳米粒的制备及其抗肿瘤活性

目的 制备莪术醇固体脂质纳米粒,并评价其抗肿瘤活性.方法 乳化超声分散法制备固体脂质纳米粒,测定粒径、Zeta电位、包封率、载药量、体外释药、光稳定性(4 500 lx,25℃).MTT法考察固体脂质纳米粒对人宫颈癌上皮细胞(Caski细胞)的抑制作用.结果 所得莪术醇固体脂质纳米粒粒径为(198.84±4.17) nm,Zeta电位为(-21.8±2.5)mV,包封率为83.27%,载药量为3.83%,36 h内累积溶出度为61.81%;体外释药符合Weibull模型(R2 =0.960 5);光照72 h后,莪术醇含有量仅降低了3.42%;对Caski细胞有较好的抑制作用,并呈量效和时效依赖性(P<0.05,P<0.01).结论 固体脂质纳米粒可明显提高莪术醇体外抗肿瘤活性.

Preparation and anti tumor activity of curcumol loaded solid lipid nanoparticles

AIM To prepare curcumol loaded solid lipid nanoparticles and to evaluate their anti tumor activity.METHODS For the solid lipid nanoparticles prepared by emulsion ultrasonic dispersion method,particle size,Zeta potential,encapsulation efficiency,drug loading,in vitro drug release and light stability(4500 lx,25℃)were determined.MTT assay was applied to investigating the inhibitory effect of solid lipid nanoparticles on human cervical cancer epithelium cells(Caski cells).RESULTS The obtained curcumol loaded solid lipid nanoparticles demonstrated the particle size,Zeta potential,encapsulation efficiency,drug loading and accumulative release rate within 36 h of(198.84±4.17)nm,(-21.8±2.5)mV,83.27%,3.83%and 61.81%,respectively,which displayed good inhibitory effect on Caski cells in dose dependent and time dependent manner(P<0.05,P<0.01).The in vitro drug release accorded with Weibull model(R2=0.9605).Exposed to 72 h lighting,curcumol content was only reduced by 3.42%.CONCLUSION Solid lipid nanoparticles can obviously enhance the in vitro anti tumor activity of curcumol.