HPLC法同时测定小儿泄泻停颗粒中9种成分

目的建立HPLC法同时测定小儿泄泻停颗粒(羌活、车前子、苍术等)中紫花前胡苷、羌活醇、异欧前胡素、京尼平苷酸、毛蕊花糖苷、异毛蕊花糖苷、苍术素醇、白术内酯Ⅱ、苍术素的含有量。方法该药物甲醇提取液的分析采用Kromasil Eternity C18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.5%冰醋酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长254、270、315 nm。结果 9种成分在各自范围内线性关系良好(r≥0.999 1),平均加样回收率96.98%~99.85%,RSD 0.87%~1.57%。结论该方法简便准确,重复性好,可用于小儿泄泻停颗粒的质量控制。

Simultaneous determination of nine constituents in Xiao’er Xiexieting Granules by HPLC

AIM To establish an HPLC method for the simultaneous content determination of nodakenin, notopterol, isoimperatorin, geniposidic acid, verbascoside, isoacteoside, atractylodinol, atracylenolideⅡ and atractylodin in Xiao’er Xiexieting Granules(Notopterygii Rhizoma et Radix, Plantaginis Semen, Atractylodis Rhizoma, etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Kromasil Eternity C18 column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.5% acetic acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelengths were set at 254, 270, 315 nm.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.999 1), whose average recoveries were 96.98%-99.85% with the RSDs of 0.87%-1.57%.CONCLUSION This simple, accurate and reproducible method can be used for the quality control of Xiao’er Xiexieting Granules.