| 禽白血病病毒斑点杂交检测方法的建立 |
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| 引用本文： | 周刚,牛成明,司昌德,韩凌霞.禽白血病病毒斑点杂交检测方法的建立[J].中国预防兽医学报,2010,32(1). |
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| 作者姓名： | 周刚 牛成明 司昌德 韩凌霞 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费项目 |
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| 摘 要： | 为建立禽白血病病毒(ALV)斑点杂交检测方法,本研究采用RT-PCR技术扩增ALV群特异性p27抗原基因的部分片段,并以纯化的p27PCR产物为模板,合成地高辛标记探针,以此建立了ALV的斑点杂交检测方法。用该方法对4份疑似感染ALV的现地病鸡组织样品和18枚鸡胚进行检测,结果表明所有样品均为阳性,而且与PCR检测结果的符合率达到100%。该方法具有良好的特异性和敏感性,适应于ALV临床大规模检测。
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Establishment of dot-blot hybridization assay for detection of avian leukosis virus |
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| ZHOU Gang,NIU Cheng-ming,SI Chang-de,HAN Ling-xia.Establishment of dot-blot hybridization assay for detection of avian leukosis virus[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(1). |
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| Authors: | ZHOU Gang NIU Cheng-ming SI Chang-de HAN Ling-xia |
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| Abstract: | The gene of avian leukosis virus p27 group specific antigen was amplified by RT-PCR and labeled with Digoxi-genin-labelled as probe. Dot-blot hybridization was developed and used to detect 4 clinical samples and 18 chicken embryos of suspected ALV infection. The result showed that all samples were detected positive by dot-blot, which was consistent with PCR re-sults. |
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| Keywords: | avian leukosis virus p27 dot blotting |
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