北京地区中国人Apo（a）多态表型的分布及其与脂质及脂蛋白的关系

为探讨Ａｐｏ（ａ）基因在中国人群中的分布特点及其与脂质及脂蛋白的关系，应用Ｗｅｓｔｅｒｎ印迹法检测１０２名北京地区中国汉族健康人群（男５７名，女４５名，年龄２１～７４岁）的Ａｐｏ（ａ）多态表型，发现：（１）Ａｐｏ（ａ）表型的分布频率在不同性别之间无明显差异；低分子量表型（包括含有Ｂ、Ｓ１和Ｓ２的全部表型）占１５．７％，其中Ｓ２＞Ｓ２Ｓ３＞Ｂ和Ｓ２Ｓ４＞Ｓ１、ＢＳ４和Ｓ１Ｓ３；高分子量表型（包括只含有Ｓ３、Ｓ４和Ｓ３Ｓ４及Ｎｕｌｌ表型）占８４．３％，其中Ｓ４＞Ｎｕｌｌ＞Ｓ３Ｓ４＞Ｓ３１纯合型占５９．８％；杂合型占１６．７％，Ｎｕｌｌ型占２３．５％；（２）Ａｐｏ（ａ）分子量大小与Ｌｐ（ａ）血浆水平呈明显负相关；与其他脂质及脂蛋白相关不明显；（３）Ｌｐ（ａ）浓度的５６．４％取决于Ａｐｏ（ａ）多态表型的变化；而其他脂质及脂蛋白则不受Ａｐｏ（ａ）多态性的影响。为冠心病的研究积累了资料。

APOLIPOPROTEIN(a) PHENOTYPES FREQUENCIES AND CONTRIBUTION TO NORMAL LIPID VARIATION IN A HEALTHY CHINESE POPULATION

Apolipoprotein(a)Apo(a)] polymorphic phenotypes in a sample of 102 unrelated healthy Chinese adults were determined by western blotting and SDS-PAGE. No significant differences in Apo(a) phenotype frequencies distribution were observed between male and female population. Apo(a) low molecular weight phenotypes constituted 15.7% of this sample, in which S2>S2S3>B, and S2S4>>S1,BS4 and S1S3; Apo(a) high molecular weight phenotypes constituted 84.3%, in which S4>Null>S3S4>S3. Homozygote type accounted for 59. 8%; heterozygote type accounted for 16. 7% and Null type 23. 5%. There was a strong inverse relationship between the apparent molecular weights of Apo(a) isoforms and plasma Lp(a) concentrations as reported previously. And no relationship was noted between Apo(a) size and other lipids or lipoproteins. Apo(a) polymorphism accounted for 56. 4% of the variability in Lp(a) concentration in Chinese, and did not explain the variabilily of other lipids or lipoproteins.