基于网络药理学探索青盐方治疗围绝经期综合征的配伍合理性研究

目的 探索青盐方治疗围绝经期综合征的配伍合理性.方法 采用网络药理学方法,分别对青盐方中君药(肉苁蓉Cistanche deserticola)、君+臣药(肉苁蓉+巴戟天Morinda officinalis)、君+臣+佐药(肉苁蓉+巴戟天+花椒Zanthoxylum bungeanum+牛膝Achyranthes ...

Compatibility rationality of Qingyan Formula in treatment of perimenopausal syndrome based on network pharmacology

Objective To explore the compatibility rationality of Qingyan Formula(青盐方)in the treatment of perimenopausal syndrome.Methods Network pharmacology was used to predict and screen the targets,function and pathways related to perimenopausal syndrome in Qingyan Formula including Sovereign(Cistanche deserticola)group,Sovereign-Minister(C.deserticola+Morinda officinalis)group,Sovereign-Minister-Assistant(C.deserticola+M.officinalis+Zanthoxylum bungeanum+Achyranthes bidentata)group and Sovereign-Minister-Assistant-Guide(Jun-Chen-Zuo-Shi)(C.deserticola+M.officinalis+Z.bungeanum+A.bidentate+Halitum)group.The characteristics and compatibility rules of this prescription in preventing and treating perimenopausal syndrome were analyzed combined with data.Results A total of 74 compounds act on 290 targets and 51 key targets of perimenopausal syndrome,mainly involving cell response,neuroendocrine,immune and other related biological processes.Pathway enrichment analysis involved estrogen receptor,PI3 K-Akt,MAPK,HIF-1,and other pathways.Conclusion Each separated compound prescription group acts on the key pathology parts-hypoestrogenia and short-term anxiety,mid-term genital atrophy and long-term osteoporosis of perimenopausal syndrome through various targets and related pathways.Each prescription group not only has different targets,but also has the same targets to act on symptoms,and plays a synergistic therapeutic role.Jun drug group includes 216 related targets,40 key targets and all major pathways in the formula.After Chen,Zuo,and Shi drugs were added in sequence,the number of targets and related pathways were increased to different degree,which indicated the importance of Jun drug-C.deserticola in the formula and the supplement of other drugs to Jun drug,and explains the compatibility rationality.