肾阳虚型慢性再障患者CD3+CD19-和CD3+CD25+T细胞与血小板的多重线性回归分析

目的 建立多重线性回归模型分析肾阳虚型慢性再生障碍性贫血（CAA）患者的总T细胞（CD3⁺CD19^-）、活化T细胞（CD3⁺CD25⁺）与血小板（PLT）的线性关系并探讨肾阳虚型CAA患者PLT数量在治疗转归中的预测因素。方法 纳入22例肾阳虚型CAA患者,收集外周血中CD3⁺CD19^-T细胞和CD3⁺CD25⁺T细胞占淋巴细胞的比例和PLT共3个参数并进行线性回归分析。结果 因学生化删除残差（SDR_1）超过3倍标准差的离群值以及强杠杆点（LEV_1） > 0.5者各有1条观测值,对这2条观测值予以删除。回归模型具有统计学意义F（2,17） = 19.479（P < 0.001）,R² = 0.696,调整R² = 0.660。纳入模型的2个自变量（CD3⁺CD19^-）和（CD3⁺CD25⁺）对PLT的影响均有统计学意义（P < 0.05）,并得到回归方程：PLT = 391.585 -5.104 × （CD3⁺CD19^-） + 23.851 × （CD3⁺CD25⁺）。结论 外周血淋巴细胞中CD3⁺CD19^-T细胞的比例和CD3⁺CD25⁺ T细胞的比例可用于有效地估计和预测肾阳虚型CAA患者在未来临床转归中PLT的值及其变化趋势,有助于临床医师及时判断和评估CAA患者在临床结局中的出血风险及出血症状对患者生存质量的影响,具有较好的预测价值和临床实践意义。

Multiple Linear Regression Analysis of CD3+ CD19-and CD3+ CD25+ T Cells and Platelets in Kidney Yang Deficiency Patients with Chronic Aplastic Anemia

Objective To establish a multiple linear regression model to analyze the linear relationship between total T cells (CD3⁺ CD19^-), activated T cells (CD3⁺ CD25⁺) and platelets (PLT) in kidney yang deficiency patients with chronic aplastic anemia (CAA) and to explore the predictive factors of PLT number in the treatment outcome of CAA in kidney yang deficiency patients.Methods 22 CAA patients with kidney yang deficiency were enrolled. 3 parameters including CD3 ⁺ CD19^-T cell ratio in peripheral blood, CD3 ⁺ CD25⁺ T cell to lymphocyte ratio and PLT were collected and analyzed by linear regression.Results Due to Studentized Deleted Residuals (SDR_1) exceeding 3 times the standard deviation and those with strong leverage points (LEV_1) > 0.5, these 2 observations were removed. The regression model had statistical significance F(2, 17) = 19.479 (P < 0.001), R² = 0.696, and adjusted R² = 0.660. The effects of the 2 independent variables included in the model on PLT were statistically significant (P < 0.05), and the regression equation was obtained: PLT = 391.585 - 5.104 × (CD3⁺CD19^-) + 23.851 × (CD3⁺CD25⁺).Conclusion The proportion of CD3⁺CD19^- T cells and CD3⁺CD25⁺ T cells in peripheral blood lymphocytes can be used to effectively estimate and predict the PLT number and its trend in the future clinical outcome of CAA patients with kidney yang deficiency, which is helpful for clinicians to timely judge and evaluate the bleeding risk and the impact of bleeding symptoms on the quality of life of CAA patients in the clinical outcome and has better predictive value and clinical significance.