| 蜻蜓凤梨叶片组织培养植株再生的研究 |
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| 引用本文： | 符运柳,徐立,李志英,黄碧兰,李克烈. 蜻蜓凤梨叶片组织培养植株再生的研究[J]. 热带作物学报, 2014, 34(9): 30-32 |
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| 作者姓名： | 符运柳 徐立 李志英 黄碧兰 李克烈 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室/海南省热带作物种质资源遗传改良与创新重点实验室 海南儋州 571737;中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室/海南省热带作物种质资源遗传改良与创新重点实验室 海南儋州 571737;中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室/海南省热带作物种质资源遗传改良与创新重点实验室 海南儋州 571737;中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室/海南省热带作物种质资源遗传改良与创新重点实验室 海南儋州 571737;中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室/海南省热带作物种质资源遗传改良与创新重点实验室 海南儋州 571737 |
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| 摘 要： | 以蜻蜓凤梨试管苗叶片为外植体进行离体再生研究。结果表明,叶片诱导直接分化不定芽的最佳培养基是MS＋BA 5.0 mg/L＋IBA 1.5 mg/L,分化率高达80%。增殖培养基为MS＋BA 0.5 mg/L＋NAA 0.2 mg/L。壮苗培养基为MS＋NAA 2.0 mg/L。生根培养基为MS＋NAA 2.0 mg/L＋AC 0.5 g/L,生根率达100%。
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| 关 键 词： | 蜻蜓凤梨 叶片 组织培养 植株再生 |
Tissue Culture and Plant Regeneration of Aechmea fasciata Leaf |
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| Affiliation: | Institute of Tropical Crop Genetic Resources / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China & Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, CATAS, Danz;Institute of Tropical Crop Genetic Resources / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China & Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, CATAS, Danz;Institute of Tropical Crop Genetic Resources / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China & Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, CATAS, Danz;Institute of Tropical Crop Genetic Resources / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China & Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, CATAS, Danz;Institute of Tropical Crop Genetic Resources / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China & Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, CATAS, Danz |
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| Abstract: | The regeneration system of Aechmea fasciata in vitro was studied with leaves as explants. The results showed the optimal medium for shoots induction was MS+BA 5.0 mg/L+IBA 1.5 mg/L with the induction frequency of 80%. The shoot multiplying medium was MS+BA 0.5 mg/L+NAA 0.2 mg/L. The elongation medium was MS+NAA 2.0 mg/L. The rooting medium was MS+NAA 2.0 mg/L+AC 0.5 g/L. The rooting frequency was 100%. |
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| Keywords: | Aechmea fasciata leaf tissue culture plant regeneration |
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