| RP-HPLC测定烧仙草不同部位中的齐墩果酸和熊果酸 |
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| 引用本文： | 冷桂华.RP-HPLC测定烧仙草不同部位中的齐墩果酸和熊果酸[J].光谱实验室,2011,28(4):2111-2114. |
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| 作者姓名： | 冷桂华 |
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| 作者单位： | 宜春学院化学与生物工程学院 江西省天然药物活性成分研究重点实验室 江西省宜春市学府路576号336000 |
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| 基金项目： | 国家“863”计划资助项目 |
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| 摘 要： | 采用超声辅助提取,建立烧仙草不同部位中齐墩果酸和熊果酸的高效液相色谱分析方法.色谱柱为Kromasil C18柱(4.6mm×250mm,5μm),流动相为甲醇∶水∶磷酸(90 ∶ 10 ∶ 0.1,V/V/V),流速0.8mL·min-1,检测波长210nm,柱温30℃.齐墩果酸在0.228-2.850μg时,与峰面...
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| 关 键 词： | 烧仙草 齐墩果酸 熊果酸 反相高效液相色谱法 光电二极管阵列检测器 |
Determination of Oleanolic Acid and Ursolic Acid in Different Parts of Mesona Chinensis Benth by RP-HPLC |
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| LENG Gui-Hua.Determination of Oleanolic Acid and Ursolic Acid in Different Parts of Mesona Chinensis Benth by RP-HPLC[J].Chinese Journal of Spectroscopy Laboratory,2011,28(4):2111-2114. |
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| Authors: | LENG Gui-Hua |
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| Affiliation: | LENG Gui-Hua(College of Chemistry and Bioengineering,Yichun University,Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines,Yichun,Jiangxi 336000,P.R.China) |
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| Abstract: | The method for the determination of oleanolic acid and ursolic acid in different parts of Mesona Chinensis Benth was established by HPLC with microwave-assisted extraction.The separation was achieved on Kromasil C18 column（4.6mm×250mm,5μm） with methanol-water-phosphoric acid（90∶10∶0.1,V/V/V） as the mobile phase at flow rate of 0.8mL·min-1,with 210nm as the detection wavelength and 30℃ as column temperature.Oleanolic acid had a good relationship in the range of 0.228—2.850μg（r=0.9998）.The recovery and RSD for oleanolic acid were 98.2% and 1.5%（n=6）,respectively.Ursolic acid had a good relationship in the range of 0.544—6.800μg（r=0.9998）.The recovery and RSD for ursolic acid were 97.8% and 1.2%（n=6）,respectively.The proposed method is accurate and simple,and that promises to be applicable for the determination of oleanolic acid and ursolic acid in Mesona Chinensis Benth. |
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| Keywords: | Mesona Chinensis Benth Oleanolic Acid Ursolic Acid RP-HPLC Photodiode Array Detector |
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