绿茶抗辐射损伤作用研究

目的 探讨绿茶对^60Coγ射线诱发小鼠辐射损伤的保护作用。方法 将小鼠35只随机分为正常对照组，辐射对照组，三个饮茶试验组，饮茶试验组动物分别饮用1．25％，2．5％，5％绿茶水浸液两周后，除正常对照动物组外，其余各组均以5Gy^60Coγ射线进行一次全身照射，并继续饮茶一周后处死全部动物，测定小鼠血清丙二醛（MDA）含量和超氧化物歧化酶（SOD）活力，股骨骨髓细胞DNA含量，有核细胞数及胸骨骨髓嗜多染红细胞微核率。结果 三个饮茶试验组小鼠血清MDA含量、骨髓嗜多染红细胞微核率显著低于辐射对照组（P＜0．05），血清SOD活力、骨髓细胞DNA含量和有核细胞数明显高于辐射对照组（P＜0．05）。结论 绿茶对辐射损作具有一定的防护作用。

Protective Effects of Green Tea on Mice with the Irradiating Damage Induced by Gamma-ray

Objective To evaluate the protective effects of green tea on mice with the irradiating damage induced by 60 Co gamma ray. Methods Thirty five mice were divided into normal control group, irradiating control group and three experiment groups. The mice of the experiment groups drank in water soluble extractives of green tea (1.25%, 2.5%, 5%) for two weeks. Then all animals were irradiated by 60 Co gamma ray except those of the normal control group. The animals of three experiment groups continued drinking the water soluble extractives of green tea for one week, after that all mice of the five groups were killed. The levels of malondiadehyde (MDA) and the activities of superoxide dismutase (SOD) in serums, the optical density (OD) value of DNA and the number of karyota of femur, the frequency of micronuclei in polychromatophilic erythrocytes of bone marrow in mice of all groups were measured. Results Compared with the measurements in the irradiating control group, the level of MDA in serums and the frequency of micronuclei in polychromatophilic erythrocytes of bone marrow in the three experiment groups were decreased significantly ( P <0.05), while the activities of SOD in serums, the OD value of DNA and the number of karyota of femur in mice of the three experiment groups were increased significantly ( P <0.05). Conclusion The water soluble extractives of green tea have protective effects on mice with the irradiating damage induced by gamma ray.