| 高比度[1,2-~2H]睾丸酮的合成 |
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| 引用本文： | 孙家秀,孙志伟,沈德存.高比度[1,2-~2H]睾丸酮的合成[J].原子能科学技术,1987(2). |
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| 作者姓名： | 孙家秀 孙志伟 沈德存 |
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| 作者单位： | 中国原子能科学研究院 北京
(孙家秀，孙志伟)，中国原子能科学研究院 北京(沈德存) |
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| 摘 要： | 睾丸酮是人体内主要的雄性激素,正确地测定其含量,对于某些疾病的诊断、治疗及研究具有重要的意义。人体内睾丸酮的含量较低,多采用放射免疫分析法及竞争性蛋白结合分析法进行测定,因此需制备1,2-~3H]睾丸酮。采用催化加成法,以1,2脱氢睾丸酮(以下均写成Δ~1睾丸酮)为前体,5%Pd-C为催化剂,二氧六环为溶剂,在氚气压为95800 Pa左右条件下进行催化加成反应,氚化时间为1.5小时,其反应式见图1。氚化产物首先采用硅胶纸薄层层析法,继而采用高压液体色谱法进行纯化,所得产品比活度达1887 GBq/mmol(51 Ci/mmol),放化纯度99%以上,已用于放免分析,用户满意,产品稳定,一年后,放化纯度仍达95.0%。
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| 关 键 词： | 睾丸酮 氚化反应 高压液体色谱 |
SYNTHESIS OF[1, 2-~3H] TESTOSTERONE OF HIGH SPECIFIC RADIOACTIVITY |
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| SUN JIAXIU,SUN ZHIWEI,SHEN DECUN Institute of Atomic Energy,P. O. Box ,Beijing.SYNTHESIS OF[1, 2-~3H] TESTOSTERONE OF HIGH SPECIFIC RADIOACTIVITY[J].Atomic Energy Science and Technology,1987(2). |
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| Authors: | SUN JIAXIU SUN ZHIWEI SHEN DECUN Institute of Atomic Energy P O Box Beijing |
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| Affiliation: | SUN JIAXIU,SUN ZHIWEI,SHEN DECUN Institute of Atomic Energy,P. O. Box 275,Beijing |
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| Abstract: | 1,2-~3H] testosterone of specific activity as high as 51 Ci/mmol, andradiochemical purity more than 99% has been prepared. The experimental condi-tions, such as reaction time, ratio of raw material catalyst and separation methodhave been studied. The product was separated by silica-paper TLC at first, then purified byhigh performance liquid chromatography. The product has good stability with apurity of 95% after one year storage. |
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| Keywords: | [1 2-~3H] testosterone Tritium reaction High performance liquid chromatography |
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