| 吲哚乙酸氧化酶和过氧化物酶关系的同工酶研究 |
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| 引用本文： | 何之常,徐乃瑜.吲哚乙酸氧化酶和过氧化物酶关系的同工酶研究[J].武汉大学学报(理学版),1994(1). |
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| 作者姓名： | 何之常 徐乃瑜 |
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| 作者单位： | 武汉大学生命科学学院 |
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| 摘 要： | 用细胞质雄性不育系小麦（Ａｅ．ｔｒｉｕｎｉｃｉａｌｉｓ）ｃｓ；（Ａｓ．Ｚｈｕｋｏｖｓｋｙｉ）ｃｓ；（Ｔ．ｔｉｍｏｐｈｅｅｖｉ）ｃｓ；（Ａｅ．ｓｐｅｌｔｏｉｄｅｓ）ｃｓ和普通小麦中国春（Ｔ．ａｅｓｔｉｖｕｍ，保持系，ｃｓ）与鄂恩１号（Ｔ．ａｅｓｔｉｖｕｍ）为材料，用Ｄａｖｉｓ和Ｒｅｉｓｆｅｌｄ不连续聚丙烯酰胺垂直板凝胶电泳技术进行吲哚乙酸氧化酶（ＩＡＡ－ｏｘｉａｓｅ）和阴、阳离子过氧化物酶同工酶分析；并用ＳＣ９１０型双波段薄层扫描仪进行扫描，记录其谱带数及强弱．结果表明阳离子过氧化物酶同工酶谱与ＩＡＡ－氧化酶同工酶谱十分相似；而阴离子过氧化物酶酶谱相差甚远．由此表明具有吲哚乙酸氧化酶活性位点的过氧化物酶，应主要是阳离子过氧化物酶．
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| 关 键 词： | 细胞质雄性不育小麦，吲哚乙酸氧化酶，离子过氧化物酶，同工酶，薄层扫描 |
STUDIED ON RELATIONSHIP THE INDOLE-3-ACETIC ACID OXIDASE AND THE PREOXIDASE IN ISOENZYME ANALYSIS |
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| He Zhichang, Xu Naiyu.STUDIED ON RELATIONSHIP THE INDOLE-3-ACETIC ACID OXIDASE AND THE PREOXIDASE IN ISOENZYME ANALYSIS[J].JOurnal of Wuhan University:Natural Science Edition,1994(1). |
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| Authors: | He Zhichang Xu Naiyu |
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| Abstract: | By using cytoplasmic male sterile (cms) line wheats(Ae triunicialis)cs,(Ae. zhukovskyi)cs, (T. timopheevi)cs,(T.speltoides)cs and normal wheat Chinese spring (T.aestivum) and, Er En No.one(T. aestivum L. Var. Er En No.1) as materials the relstionship between indole-3-acetic acid and perokidase(cationic peroxidase,cpox and anionic peroxidase,apox) in isozymogram was Studied with discontinual vertical polyacrylamide gel electrophoresis and thin layer scanning technique. ResultS showed that the relationship of cationic peroxidase to indoleacetichcid oxidase is more close. But anionic peroxidase have no relation to IAA-oxidase. Because it will be seen that cationic peroxidase could mainly have an activity of indole-3-acetic acid oxidase. |
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| Keywords: | cytoplasmic male sterile wheat indole-3-acetiC acid oxidase cationic (or anionic)peroxidase isoenzyme thin layer scanning |
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