痛泻要方对肠易激综合征大鼠血浆及肠黏膜CGRP影响的实验研究

目的：探讨肠易激综合征（irritable bowel syndrome）的发病机制以及痛泻要方煎剂治疗肠易激综合征内脏高敏感性模型大鼠的机制。方法：选用清洁级新生sD大鼠，采用结肠慢性刺激法加夹尾刺激法造模。造模成功后，将实验动物分组为空白对照组（A组）、模型对照组（B组）、中药低剂量组（C组）、中药中剂量组（D组）、中药高剂量组（E组）、西药奥替溴铵（F组）。A组和B组以生理盐水按4ml／100g（40g／kg／d）灌胃，C、D、E、F组分别以低剂量痛泻要方煎剂组按0．4ml／100g（4g／kg／d）、中剂量痛泻要方煎剂组按1．2ml／100g（12g／kg／d）、高剂量痛泻要方煎剂组按4ml／100g（40g／kg／d）灌胃、西药奥替溴铵组按4ml／100g（150g，kg／d）灌胃，每天两次，连续30天。结果：痛泻要方高剂量组能显著增加血浆和结肠组织CCRP含量，痛泻要方低剂量组、中剂量组与西药组均不能。结论：痛泻要方可能是通过增加模型大鼠血浆和结肠组织CGRP含量来实现的。

Impacts of Tongx ieyaofang Decoction on Intestinal CGRP in RatModel with Visceral Hyper sensitivity inIrritable Bowel Syndrome

Objective：To explore the pathogenesis of irritable bowel syndro--me（IBS） and the mechanism of curing experimental rat model of IBS in Tongxieyaofang decoction. Methods： Model rats of IBS, which adopt the model chronic rectal irritation and stimulation with forceps clip on tails in the neonatal rats. On the base of above successful model, the rates were randomly divided into six groups： the normal control group（Group A）,the model group（Group B）,the low dosage of Tongxie Yaofang decoction（Group C）,the moderate dosage of Tongxie Yaofang decoction（Group D）,the high dosage of Tongxie Yaofang decoction（Group E）,the Spasmomen control group（Group F）.Gmup A and Group B received oral administration in 4ml/100g（40g/ kg/d） of NS, Group C received oral administration in 0.4ml/100g/（4g/kg/d）, Group D received oral administration in 1.2ml/100g（12g/kg/d）, Group E received oral administration in 4ml/100g（40g/kg/d）, Group F received oral administration in 4ml/100g（40g/kg/d） twice everyday for one month. Results： he level of CGRP in plasma and mueosa of the model group rats descends significantly.The high dosage of Tongxieyaofang group were able to make it increase significantly.But there is no mutation in the low dosage of Tongxieyaofang group,the middle dosage of Tongxieyaofang group and the group of Shibamin.Conclusion： The possible mechanism of Tongxieyaofang ：It makes CGRP in plasma and mucosa increased significantly.