U100倍频双波长脉冲激光对兔膀胱黏膜的损伤效应

目的 观察不同频率、不同脉冲的U100倍频双波长脉冲激光对家兔膀胱黏膜的损伤效应。方法 新西兰兔6只，避开膀胱三角区随机取四点进行实验，予置激光输出频率分别为3、10Hz，脉冲输出500、1000次，垂直接触照射膀胱黏膜，通过肉眼、光镜观察靶组织损伤的形态学改变。结果 激光照射后，兔膀胱黏膜肉眼未见明显穿孔，光镜下可见激光照射处膀胱黏膜上皮细胞不同程度的脱落，固有层出血等病理改变，无肌层平滑肌细胞断裂等严重损伤；显示脉冲次数为500的损伤主要限于膀胱黏膜上皮浅层，脉冲次数为1000的损伤累及上皮全层。结论 频率为10Hz，脉冲1000次的U100倍频双波长脉冲激光照射膀胱黏膜不会引起穿孔，但可引起上皮细胞脱落，固有层出血等病理改变。激光损伤程度与照射脉冲次数相关。

Injury of Rabbit Bladder Epithelium Induced by U100 Laser

Objective To study the effect of U100 laser on rabbit bladder epithelium with different frequency and output.Methods U100 Laser with different frequency(3Hz and 10Hz)and output(500pulses and 1000pulses) was acted on rabbit bladder epithelium.Morphological changes of target tissue were observed.Results There was no perforation observed by eyes.By microscopy,it could be observed that the epithelium desquamated with different degree,and hemorrhage under epithelium.There was no rupture of all the muscle cells.It showed that exterior epithelium desquamated with 500 pulses and interior epithelium desquamated with 1000 pulses.Conclusions U100 Laser could not cause muscle cells rupture,but it may cause injury such as epithelium desquamation and hemorrhage.The degree of the injury is related to the dose.