胰高血糖素样肽-2对幼鼠肠缺血-再灌注损伤的保护作用

目的 研究胰高血糖素样肽2(GLP-2)对肠缺血-再灌注(I-R)幼鼠肠屏障功能的保护作用.方法 采用肠I-R模型,将24只雄性Wistar幼鼠随机均分为假手术(C组)、肠I-R损伤(B组)和肠I-R损伤+GLP-2保护(A组)三组.光镜下观察小肠黏膜形态学改变,检测肠系膜淋巴结的细菌菌落情况、肠黏膜丙二醛(MDA)、血清D-乳酸、内毒素和肿瘤坏死因子α(TNF-α)水平的变化.结果 B组肠黏膜损伤最明显,Chiu's评分、肠系膜淋巴结细菌菌落计数、肠黏膜MDA、血清D-乳酸、内毒素和TNF-α水平均显著高于C组(P<0.01);与B组相比,A组肠黏膜损伤明显减轻,且Chiu's评分、肠系膜淋巴结细菌菌落计数、肠黏膜MDA、血清乳酸、内毒素和TNF-α水平显著降低(P<0.05).结论 GLP-2能够减轻幼鼠肠I-R损伤、保护肠黏膜屏障、减轻细菌/内毒素易位、减少氧自由基和炎性介质的产生和释放.

Protective effects of glucagon-like peptide 2 on intestinal ischemia-reperfusion injury of young rats

Objective To explore the protective effects of glucagon-like peptide 2 (GLP-2) on intestinal ischemia-reperfusion(I-R)injury of young rats. Methods Using the rat model of intestinal ischemia-reperfusion, 24 young male Wista rats were randomly assigned to three groups of sham operation( group C), I-R( group B) and I-R+GLP-2 (group A) with 8 rats each. Intestinal pathology was examined under light microscope. Bacterial colony of mesenteric lymph node(MLN), intestinal mucosal malondialdehyde(MDA), serum D-lactate, endotoxin, and tumor necrosis factoe(TNF)-α were studied. Results The intestinal injury was significant in group B, and Chiu's scores, bacterial colony counts of MLN, intestinal mucosal MDA, serum D-lactate, endotoxin, and TNF-α were also significantly higher in group B than those in group C (P<0. 01), which were significantly lower in group A than those in group B (P<0. 05). Conclusion GLP-2 attenuates intestinal I-R injury, protects intestinal mucosal barrier, reduces bacterial/endotoxin translocation, inhibits the release of oxygen free radicles and inflammatory cytokines.