牛轮状病毒受体分析

目的分析牛轮状病毒受体的理化性质及该受体在气管粘膜组织的分布。方法采用ＳＤＳ－聚丙烯酰胺凝胶电泳法（ＳＤＳ－ＰＡＧＥ）、酶消化法、固相分析法对纯化的牛轮状病毒受体进行分析，以免疫组化法检测了新生牛气管粘膜细胞表面牛轮状病毒受体。结果牛轮状病毒受体ＳＤＳ－ＰＡＧＥ分子量为４８０００，该受体对胰蛋白酶及ＰｒｏｎａｓｅＥ蛋白酶敏感，经Ｃｌｏｓｔｒｉｄｉｕｍｐｅｒｆｒｉｎｇｅｎｓ及Ａｒｔｈｒｏｂａｃｔｅｒｕｒｅａｆａｃｉｅｎｓ神经氨酸酶作用后，受体失去结合病毒的生物学活性。免疫组化试验结果为阳性。结论实验结果表明牛轮状病毒受体是结构复杂的糖蛋白，唾液酸残基是受体与牛轮状病毒作用位点的组成部分。新生牛气管粘膜细胞表面有牛轮状病毒受体存在。

Analysis of the receptor for bovine rotavirus

Objective We studied the physical and chemical characteristics of the receptor for bovine rotavirus and the distribution of the receptor on the tracheal mucous membrane. Methods The methods of SDS polyacrylamide gel electrophoresis,enzyme digestion assay and solid phase assay were used to analyze the purified receptor for bovine rotavirus.The receptor on the cell surface of the tracheal mucous membrane of neonatal bovine was verified by immunohistchemistry method. Results The receptor molecular weight was 48kD on SDS polyacrylamide gel electrophoresis.It was sensitive to trypsin and Pronase E.Treated by the neuraminidase from Clostridium perfringens or Arthrobacter ureafaciens, the receptor lost its biological activity of binding virus.The assay result of immunochemistry was positive. Conclusions All these results above showed that the receptor for bovine rotavirus was a kind of glycoprotein with a complex structure.Sialic acid remnant is infected to be a main part of the receptor site reacting with bovine rotavirus.The receptor for bovine rotavirus has existed on the cell surface of the tracheal mucous membrane of neonatal bovine.