基于SSR标记的鸢尾杂交和诱变育种材料遗传多样性分析

为加快路易斯安那鸢尾育种的进程,以5个路易斯安那鸢尾品种为试验材料,开展杂交和60Co-γ辐射诱变育种.基于短茎鸢尾和暗黄鸢尾的基于表达序列(EST)开发的微卫星标记(SSR)标记,选择其中14对SSR标记用于路易斯安那鸢尾品种、杂交后代和诱变材料共40份材料的遗传多样性分析.结果表明,5个路易斯安那鸢尾品种的杂交后代...

Genetic Diversity Analysis of Iris Hybrid and Mutagenic Breeding Materials Based on SSR Markers

In order to speed up the breeding process of Louisiana iris, hybridization and ⁶⁰Co-γ radiation mutagenesis were carried out on five Louisiana iris varieties. Based on the EST-SSR markers of I.brevicaulis and I.fulva, 14 pairs of SSR markers were selected for genetic diversity analysis on Louisiana iris varieties, their hybrid offspring and mutagenic materials. The results showed that there was little genetic difference between the offspring of five Louisiana iris varieties and their maternal plants. However, after irradiation with ⁶⁰Co-γ, great genetic differences from their parents were observed rays for five Louisiana iris cultivars. The survival rate of tissue culture plants was significantly negatively correlated with the dose of ⁶⁰Co-γ mutagenesis. And the survival rates of different varieties under treatments of 2.5, 5, 10, 20 and 40 Gy were 20.77%~24.99%, 13.33%~17.89%, 10.11%~15.71%, 3.33~10.14% and 1.11%~2.77% respectively. The phenotypic traits of the mutagenic materials could be observed via tissue culture rapid propagation system. In this study, in vitro culture combined with ⁶⁰Co-γ radiation mutation breeding was carried out for the first time on Louisiana iris, and 10 pairs of SSR markers were screened for identifying Louisiana iris varieties, hybrid offspring and mutagenic materials were screened. The research provide theoretical basis for Louisiana iris hybrid breeding, radiation mutation breeding and SSR-based germplasm identification.