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| SELDI-TOF-MS筛查乳腺癌患者血清特异性蛋白的探讨 | |
| 引用本文： | 刘彩刚,路平,赵祥,顾劲松,刘金龙,庞葵,朱晓宣,张瑞山.SELDI-TOF-MS筛查乳腺癌患者血清特异性蛋白的探讨[J].中国肿瘤临床,2007,34(13):728-730. |
| 作者姓名： | 刘彩刚 路平 赵祥 顾劲松 刘金龙 庞葵 朱晓宣 张瑞山 |
| 作者单位： | 1. 中国医科大学第一附属医院肿瘤研究所,沈阳市,110001 2. 辽宁省肿瘤医院胸外科 3. 湖北省宜昌市长江三峡医院 |
| 摘 要： | 目的：应用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）技术寻找乳腺癌患者血清特异性蛋白.方法：应用弱阳离子蛋白质芯片（WCX2）及SELDI-TOF-MS技术检测12例正常人、10例乳腺良性病患者和22例乳腺癌患者血清中蛋白的相对含量.结果：三组样本质荷比在2000～30000 Da间3939.314Da与3960.478Da的2个蛋白峰有显著差异.结论：SELDI-TOF-MS技术对乳腺癌早期诊断和特异性肿瘤标记物的筛选等方面具有一定价值,值得推广. |
| 关 键 词： | 乳腺肿瘤 弱阳离子蛋白质芯片 表面增强激光解吸电离飞行时间质谱 |
| 修稿时间： | 2006-10-312007-02-02 |
The Study of Searching for the Serum Proteomic Patterns of Breast Cancer by SELDI-TOF-MS |
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| Liu Caigang, Lu Ping, Zhao Xiang.The Study of Searching for the Serum Proteomic Patterns of Breast Cancer by SELDI-TOF-MS[J].Chinese Journal of Clinical Oncology,2007,34(13):728-730. | |
| Authors: | Liu Caigang Lu Ping Zhao Xiang |
| Affiliation: | Oncology Department, First Hospital, China Medial University, Shenyang |
| Abstract: | Objective: To detect the serum proteomic patterns in breast cancer patients by surface enhanced laser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS). Methods: The relative contents of serum proteins of 12 healthy people,10 patients with benign breast diseases and 22 patients with breast cancer were detected by WCX2 chip and SELDI-TOF-MS. Results: At the M/Z values range from 2000Da to 30000Da,the contents of two proteins (3939.314Da and 3960.478Da)had significant difference in the three groups. Conclusions: SELDI-TOF-MS shows great potentiality for breast cancer in early diagnosis and screening of tumor biomarkers,and deserves generalization. |
| Keywords: | Breast neoplasms Weak cation exchanger protein chip Surface enhanced laser desorption/ionization time of flight mass spectrometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
