神经生长因子对急性脑梗死大鼠神经功能和一氧化氮合酶含量的影响

目的 观察神经生长因子（NGF）对实验性急性脑梗死大鼠的疗效，初步探讨可能的机制。方法 将24只制备成的脑梗死模型大鼠随机分为3组，分别用神经生长因子（NGF）、胞磷胆碱钠（CS）和生理盐水（NS）治疗，于治疗前后检查动物的神经病学评分改变；再将55只大鼠随机分为实验组（25只）、对照组（25只）和正常组（5只），实验组急性脑梗死后即刻肌肉注射NGF，对照组注射等量NS。对照组和实验组大鼠分别在脑梗死后1h、3h、6h、12h、24h断头取脑，检测脑组织中一氧化氮合酶（NOS）含量。结果 与治疗前比较，NGF组和CS组大鼠治疗后神经病学评分均低于NS组（P〈0．05）；脑梗死大鼠梗死区脑组织中NOS活性在梗死后1h、3h较正常组明显升高（P〈0．01）；实验组大鼠NOS含量在脑梗死后1h、3h、6h较对照组明显下降（P〈0．01）。结论 NGF可明显促进急性脑梗死大鼠神经功能的恢复，机制之一可能是通过抑制急性脑梗死后NOS的活性起到保护损伤脑神经元的作用。

Effects of nerve growth factor on the nerve function and nitric oxide synthase to acute cerebral embolism in rats

ObjectiveTo observe the curative effects of nerve growth factor (NGF) on experimental acute cerebral thromboemblia rats and study the mechanisms preliminarily.Methods24 model rats were randomly divided into three groups treated respectively with NGF, citicoline sodium (CS) and normal saline (NS) for 20 days, and the neurological grades of animals were observed before and after treatment. Then, 55 rats were randomly divided into three groups: the treated group (25 model rats, treated with NGF), control group (25 model rats, treated with NS) and normal group (5 normal rats, without treatment), the levels of nitric oxide synthase (NOS) of all animals were measured at 1 h, 3 h, 6 h, 12 h and 24 h after acute cerebral thromboemblia established.ResultsThe neurological grades of both NGF and CS treated groups were significantly lowered after treatment compared with NS control group ( P<0.05). NOS levels of cerebral thromboemblia areas were higher than that in the control group 1 hour, 3 hours after acute cerebral thromboemblia, the levels of NOS in NGF treatment group were obviously lower than that in the control group post-traumatic 1 hour, 3 hours and 6 hours.ConclusionNGF can accelerate the nervous function recovery of the rat with acute cerebral thromboemblia, the mechanisms is that NGF prohibits neurotoxicity of NOS.