添加氧化铝对灰树花菌丝体生长及多糖产量的影响 |
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引用本文: | 陈潇筱,杨焱,崔凤杰,孙文敬,刘伟民. 添加氧化铝对灰树花菌丝体生长及多糖产量的影响[J]. 食用菌学报, 2016, 0(1): 42-46. DOI: 10.16488/j.cnki.1005-9873.2016.01.009 |
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作者姓名: | 陈潇筱 杨焱 崔凤杰 孙文敬 刘伟民 |
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作者单位: | 1. 江苏大学食品与生物工程学院,江苏镇江,212013;2. 上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发中心,上海 201403 |
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基金项目: | 国家自然科学基金(31101269),中国博士后基金第六批特别资助项目(2013T60648),江苏省高等学校大学生实践创新训练计划项目(201410299056Y),江苏大学“青年骨干教师培养工程”青年学术带头人培育计划资助 |
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摘 要: | 考察添加不同浓度的氧化铝(粒径为200~300目)对灰树花(Grifola frondosa)发酵菌丝体生长及多糖(胞内和胞外粗多糖)产量的影响。结果表明,在试验添加范围内,灰树花菌球直径随氧化铝添加浓度的增加而显著变小,当添加氧化铝浓度为20 g/L时,游离菌丝体明显增多,且菌球主要表现为S型(D<0.5 cm,D:菌体当量直径,与对象具有相等面积的圆形的直径),其中L(D≥1.5 cm)、M(0.5 cm≤ D<1.5 cm)和 S 型菌丝体的比例分别为4.9%、24.3%和70.8%;添加3 g/L氧化铝时灰树花菌丝体生物量最高,达到5.81 g/L,比空白组提高了2.7倍;添加20 g/L氧化铝时灰树花胞外多糖产量达到最大,为8.46 g/L,为空白组的1.7倍左右,而菌丝体多糖产量以添加0.1 g/L氧化铝时最高,为65.6 mg/g。
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关 键 词: | 灰树花 氧化铝 菌丝体形态 胞外多糖 |
Effect of Aluminum Oxide on Grifola frondosa Mycelial Biomass,Pellet Diameter and Polysaccharide Production |
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Abstract: | Mycelial biomass, pellet diameter, exo-polysaccharide and mycelium-associated polysaccharide production in submerged cultures of Grifola frondosa CS-15 grown in media (g/L,30.0 glucose,6.0 peptone,3.0 KH2 PO3 ,1.5 MgSO4·7H2 O)supplemented with different concentrations (0.1 g/L to 20.0 g/L) of aluminum oxide were determined. Mycelial pellet diameters decreased significantly with increasing concentrations of aluminum oxide, and the size distribution of the highly dispersed pellets in cultures containing 20.0 g/L aluminum oxide was as follows:L fraction (D≥1.5 cm)4.9%,M fraction (0.5 cm≤D<1.5 cm)24.3%,S fraction (D<0.5 cm)70.8%.Highest mycelial biomass levels (5.81 g/L),and yields of exo-polysaccharide (8.46 g/L)and mycelium-associated polysaccharide (65.6 mg/g)were recorded in cultures supplemented with 3.0 g/L,20.0 g/L and 0.1 g/L aluminum oxide,respectively. |
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Keywords: | Grifola frondosa aluminium oxide mycelial morphology exo-polysaccharide |
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