| BLU基因慢病毒表达载体的构建 |
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| 引用本文： | 陈刚建,张宝,李燕,朴仲贤,陈松建,刘俊晓,黄晋荣,郑桂娜,郭勇勇,霍霞. BLU基因慢病毒表达载体的构建[J]. 汕头大学医学院学报, 2008, 21(4) |
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| 作者姓名： | 陈刚建 张宝 李燕 朴仲贤 陈松建 刘俊晓 黄晋荣 郑桂娜 郭勇勇 霍霞 |
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| 作者单位： | 1. 汕头大学医学院分析细胞学实验室,广东,汕头,515041
2. 南方医科大学生物化学教研室,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:构建BLU基因慢病毒表达载体。方法:采用PCR技术从人脐带来源的间充质干细胞中扩增出BLU基因,将其接入慢病毒载体(pSL6-IRES-EGFP)中,经菌落PCR、酶切和测序鉴定,然后转染293T细胞观察表达情况。结果:PCR、酶切和测序鉴定克隆了重组子(pSL6-BLU-IRES-EGFP),并在293T细胞中成功表达BLU基因。结论:成功地克隆了含有BLU基因的慢病毒表达载体。
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| 关 键 词： | BLU基因 慢病毒载体 |
Construction of BLU Gene Recombinant Lentivirus Vector |
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| CHEN Gang-jian,ZHANG Bao,LI Yan,PIAO Zhong-xian,CHEN Song-jian,LIU Jun-xiao,HUANG Jin-rong,ZHENG Gui-na,GUO Yong-yong,HUO Xia. Construction of BLU Gene Recombinant Lentivirus Vector[J]. Journal of Shantou University Medical College, 2008, 21(4) |
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| Authors: | CHEN Gang-jian ZHANG Bao LI Yan PIAO Zhong-xian CHEN Song-jian LIU Jun-xiao HUANG Jin-rong ZHENG Gui-na GUO Yong-yong HUO Xia |
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| Affiliation: | CHEN Gang-jian1,ZHANG Bao2,LI Yan1,PIAO Zhong-xian1,CHEN Song-jian1,LIU Jun-xiao1,HUANG Jin-rong1,ZHENG Gui-na1,GUO Yong-yong1,HUO Xia1 |
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| Abstract: | Objective:To construct the lentivirus vector containing BLU gene.Methods:The full-length BLU gene was amplified by PCR technique from the total RNA of the umbilical mesenchymal stem cells.BLU gene was subcloned into pSL6IRES-EGFP vector.The identification was performed by analysis of restricting enzyme digestion and DNA sequence.Results:pSL6-Blu-IRES-EGFP was constructed successfully and identified by PCR,digestion and sequencing.The recombinant could express in 293T cell.Conclusion:The recombinant lentivirus vector containing BLU gene is cloned successfully. |
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| Keywords: | BLU gene lentivirus vector |
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