白藜芦醇联合乌司他丁治疗急性胰腺炎的疗效分析

目的：观察白藜芦醇联合乌司他丁对大鼠急性胰腺炎（AP）的治疗效果。方法：将250只雄性Wister大鼠随机分为5组，分别为假手术（A）组、急性胰腺炎组（B组）、白藜芦醇组（C组）、乌司他丁组（D组）和白藜芦醇联合乌司他丁治疗组（C＋D组）。制备各组AS大鼠模型，观察各组腹水量及血淀粉酶、脂肪酶、TNF-α、NF—κB、丙二醛（MDA）及超氧化物歧化酶（SOD）水平，并观察胰腺病理改变。结果：B组与A组相比，SOD水平明显降低（P〈0．05），MDA水平明显升高（P〈0．05）；C、D及C＋D组与B组相比，血清SOD水平明显升高（P〈0．05），MDA水平明显降低（P〈0．05）；C＋D组与C组、D组相比，血清SOD水平明显升高（P〈O．05），血清MDA水平明显降低（P〈0．05）。与B组比较，C和D组血淀粉酶、脂肪酶、TNF-α、病理学评分均降低，细胞超微结构损伤减轻，有显著性差异（P〈0．05或P〈0．01）。C＋D组上述指标改善较C组和D组更明显，差异有显著性（P〈0．05）。结论：乌司他丁、白藜芦醇通过升高SOD保护性因子、降低MDA损伤性因子，对急性胰腺炎有治疗作用，尤其在乌司他丁与白藜芦醇合用时效果更显著。

Resveratrol and Ulinastatin for Acute Pancreatitis

Objective： To observe the therapeutic effects ofresveratrol and ulinastatin on acute pancreatitis （AP） of rats. Methods： A total of two hundred and fifty male Wister rats were randomly divided into sham group（A group ）, AP model group（B group）, resveratrol （C group）, ulinastatin group （D group） and resveratrol combined with ulinastatin group （C＋Dgroup）. Plasma concentrations of amylase and lipase, tumor necrosis factor-α （ TNF-α ）, nuclear factor-κ B （NF-κB）,blood levels of superoxide dismutase （SOD） and malondialdehyde （MDA） were determined. The pathological changes of pancreas were studied under light and electron microscopes. Results： SOD activity was decreased and the concentrations of amylase, lipase, TNF -α , MDA level in AP rats, while the pathological change of pancreas was severe. Compared with B group, the plasma concentrations of amylase and lipase, TNF-ct , the pathological scores and MDA in C group and D group were decreased, while SOD activity was increased and ultrastructural injury wasmarkedly attenuated; Conclusion： Resveratrol combined with ulinastatin is more effective than either of them used alone in treatment of severe AP.